哮喘儿童IL-17A rs2275913基因多态性的临床意义

Clinical significance of IL-17A rs2275913 gene polymorphism in childhood with asthma

目的探讨白细胞介素17A(IL-17A)rs2275913基因多态性与哮喘患儿转化生长因子β1(TGF-β1)和免疫球蛋白E(IgE)表达水平的临床意义及哮喘易感性的关系。方法随机抽取2018年6月至2020年6月连云港市第一人民医院收治的哮喘患儿45例为哮喘组,随机选取同时期健康体检儿童45例为对照组。采用限制性片段长度多态性聚合酶链反应(PCR-RFLP)技术检测两组儿童的IL-17A rs2275913基因多态性,采用酶联免疫吸附试验(ELISA)检测两组儿童外周血IL-17A和TGF-β1水平,采用化学发光法检测IgE水平。分析IL-17A rs2275913基因多态性与TGF-β1、IL-17A、IgE水平及哮喘的相关性。结果IL-17A rs2275913位点含有3种单核苷酸多态性,经Hardy-Weinberg遗传平衡验证显示,两组组内IL-17A rs2275913基因位点的预测与实测分布差异均无统计学意义(P>0.05)。两组基因型频率比较差异有统计学意义(χ^(2)=7.95,P<0.05),哮喘组等位基因A频率高于对照组(χ^(2)=7.50,P<0.05)。对照组TGF-β1、IgE和IL-17A水平均低于哮喘组(t值分别为26.83、48.34、12.87,P<0.05);哮喘组基因型AA和GA的TGF-β1、IgE和IL-17A水平均高于基因型GG(F值分别为4.59、8.49、114.01,P<0.05)。二元Logistic回归分析显示,基因型GA(95%CI:1.145~2.974)、基因型AA(95%CI:1.327~4.531)和等位基因A(95%CI:1.248~3.746)的携带者患哮喘的可能性是基因型GG患儿的1.831、2.431和2.146倍(P<0.05)。结论IL-17A rs2275913基因位点基因型GA和AA携带患儿表达的TGF-β1、IgE和IL-17A水平增高,IL-17A rs2275913基因位点的等位基因由G突变为A时,增加了儿童哮喘发生率,等位基因A是哮喘的易感因子。

Objective To explore the relationship between interleukin 17 A(IL-17 A)rs2275913 gene polymorphism and the clinical significance of transforming growth factorβ1(TGF-β1)and immunoglobulin E(IgE)expression in children with asthma and the relationship between asthma susceptibility.Methods A total of 45 children with asthma admitted to Lianyungang first people′s hospital from June 2018 to June 2020 were randomly selected as the asthma group,and 45 children who was healthy physical examination during the same period were randomly selected as the control group.Restriction fragment length polymorphism polymerase chain reaction(PCR-RFLP)technology was used to detect IL-17 A rs2275913 gene polymorphism of the two groups.Enzyme linked immunosorbent assay(ELISA)was used to detect peripheral blood TGF-β1 and IL-17 A of the two groups,and the chemiluminescence method was used to detect IgE level.The correlation between IL-17 A rs2275913 gene polymorphism and TGF-β1,IL-17 A,IgE and asthma were analyzed.Result IL-17 A rs2275913 site contained three single nucleotide polymorphisms.The Hardy-Weinberg genetic balance verification showed that the difference between the predicted value and the measured value of IL-17 A rs2275913 locus was not statistically significant(P>0.05).There was a statistically significant difference in the genotypes frequency between the two groups(χ^(2)=7.95,P<0.05).The frequency of type A allele in the asthma group was higher than that in the control group(χ^(2)=7.50,P<0.05).The levels of TGF-β1,IgE and IL-17 A in the control group were lower than those in the asthma group(t=26.83,48.34 and 12.87,respectively,P<0.05).The levels of TGF-β1,IgE and IL-17 A of genotypes AA and GA in the asthma group were higher than those of genotype GG(F=4.59,8.49 and 114.01,respectively,P<0.05).Binary Logistic regression analysis showed that genotype GA(95%CI:1.145-2.974)genotype AA(95%CI:1.327-4.531)and allele A(95%CI:1.248-3.746)carriers were 1.831,2.431 and 2.146 times more likely to develop asthma than children with genotype GG(P<0.05).Conclusion IL-17 A rs2275913 gene locus genotype GA and AA carriers increased levels of TGF-β1,IgE and IL-17 A in children.When the allele of IL-17 A rs2275913 gene locus is mutated from G to A,the incidence of childhood asthma is increased.Allele A is a susceptibility factor for asthma.