miR-146a和miR-149多态性与子痫前期易感性

The correlations of miR-146a and miR-149 polymorphisms with the susceptibility of preeclampsia

目的探讨miR-146a和miR-149与子痫前期易感性的关系。方法收集112例子痫前期和105例正常妊娠妇女的外周静脉血。采用PCR-RFLP技术对miR-146a rs2910164和miR-149 rs2292832位点的等位基因和基因型的频率分布进行检测,logistic回归方法分析其与子痫前期的关系。结果miR-146a rs2910164 GC和CC基因型频率在子痫前期组和对照组之间无统计学差异。除了隐性基因型外,miR-146a rs2910164多态性的显性基因型(GC+CC vs GG:P=0.039)和等位基因型(G vs C:P=0.038)与子痫前期的易感性相关。rs2910164 GC和CC基因型增加重度子痫前期的患病风险(P=0.008,P=0.0026)。rs2910164多态性的显性基因型(GC+CC vs GG:P=0.003)可增加重度子痫前期的发病风险。子痫前期组和对照组miR-149 rs2292832基因型频率相比较,差异无统计学意义(P>0.05),并且与子痫前期或重度子痫前期的风险无相关性(P>0.05)。结论miR-146a rs2910164 G/C多态性与子痫前期和(或)重度子痫前期风险相关,miR-149 rs2292832与子痫前期易感性无关。

Objective To investigate the correlations of miR-146a rs2910164 and miR-149 rs2292832 nucleotide polymorphisms with the susceptibility of preeclampsia(PE).Methods The blood samples from 112 PE and 105 normotensive pregnant women were collected.MiR-146a and miR-149 polymorphisms were genotyped in blood samples using PCR-RFLP method.The association of miR-146a rs2910164 and miR-149 rs2292832 nucleotide polymorphisms with the risk of PE was assessed by logistic regression analysis.Results There was no significant association of miR-146a rs2910164 GC and CC genotypes with PE(P>0.05).However,the miR-146a rs2910164 polymorphism was significantly correlated with an increased risk of PE in dominant(GC+CC vs.GG:P=0.039)and allelic(G vs.C:P=0.038),but not recessive models.Moreover,the GC and CC genotypes had significantly higher risk of severe PE(P=0.008 and P=0.0026,respectively),and miR-146a rs2910164 polymorphism could increase risk of severe PE in dominant models(GC+CC vs.GG,P=0.003).No evidence of an association was shown between miR-149 rs2292832 polymorphisms and PE or severe PE risk in allelic,dominant or recessive models.Conclusion The polymorphism of miR-146a rs2910164 but not miR-149 rs2292832 is probably associated with increased risk of PE and/or severe PE.