T细胞受体(TCR)库及其高通量测序常用分析工具

T cell receptor(TCR)repertoire and common analytical tools for high-throughputs equencing

T细胞受体(TCR)是T细胞识别抗原的关键部分,个体中所有的TCR总和称为TCR库。研究机体健康或患病状态下TCR库的组成及特征有助于我们更好地理解适应性免疫。目前已有大量针对高通量数据的分析工具,分析内容包括基本组成分析和下游功能分析等。抗原特异性T细胞数据库的建立为临床研究提供基础,对TCR库功能特征的分析是重点与难点,但现阶段已发布的下游分析工具还有待更多的实践与验证。我们总结了几种常用的TCR库分析工具,为非生物信息专业的科研人员选择分析工具提供参考。

T cell receptor(TCR)is an integral part of T cell recognition antigen,and the sum of TCR of all T cells in an individual is called the TCR repertoire.Understanding the composition and characteristics of the TCR repertoire in healthy and pathological state helps us to gain an insight into adaptive immunity.At present,many analysis tools for high-throughput data have been developed,including basic composition analysis and downstream function analysis.The database of antigen-specific T cells established by researchers is fundamental to clinical research.The analysis of the functional characteristics of the TCR repertoire is critical to the understanding of TCR repertoire.However,the existing downstream analysis tools still requires further application and verification.This paper reviewed several commonly used TCR repertoire analysis tools,which hopefully can provide a reference for non-bioinformatics researchers in choosing analysis tools.