口蹄疫、猪瘟、伪狂犬病疫苗联合免疫临床效果评估

Clinical Evaluation of Combinational Vaccines for Foot-and-Mouth Disease,Classical Swine Fever and Pseudorabies

本试验旨在评估口蹄疫、猪瘟、伪狂犬病混合疫苗在猪群中的免疫效果。先将商业口蹄疫、猪瘟、伪狂犬病疫苗混合,而后对混合疫苗的物理性状、疫苗毒价变化进行测定。从猪场选取保育猪(2500头)、后备母猪(1000头)、经产母猪(1000头),分成5组(A、B、C、D、E),给予注射疫苗。猪群的特异性抗口蹄疫、猪瘟、伪狂犬病抗体效价通过ELISA试剂盒检测。结果显示,混合疫苗物理性状稳定,免疫猪群产生了较高的抗口蹄疫、猪瘟、伪狂犬病特异性抗体,特别是二免后抗体效价更高,免疫持续期不受影响。由于免疫副反应率比较低,猪只免疫次数以及人猪接触频率显著减少,混合疫苗为口蹄疫、猪瘟、伪狂犬病的防控提供了有效途径。

The purpose of this study was to evaluate the effectiveness of combinational vaccines for preventing foot-and-mouth disease(FMD),classical swine fever(CSF),and pseudorabies(PR)in pig farms.Vaccines for FMD,CSF and PR were obtained commercially and combined,and the physical properties and potency of the combinational vaccines were assessed.Nursery pigs(2500),gilts(1000),and multiparous sows(1000)were selected from pig farms and divided into 5 groups(A,B,C,D,E),and injection with the combinational vaccines.Specific antibody titers to FMD,CSF,and PR were detected by ELISA kits.The results showed that combinational vaccines were physically stable,and pigs immunized with the combinational vaccines generated high titers of specific antibodies to FMD,CSF,and PR,even higher after second dose.With relatively few side effects,infrequent doses,and reduced contact with human personnel,combinational vaccines offer efficient approach to prevent FMD,CSF,and PR in pig farms.