山慈菇的化学成分研究

Studies on the Chemical Constituents of Cremastra appendiculata

本文对药材山慈菇[Cremastra appendiculata(D.Don)Makino]的化学成分进行研究。采用硅胶柱色谱、Sephadex LH-20和半制备高效液相、结晶、重结晶等技术对山慈菇80%乙醇提取物进行分离,并依据所得单体化合物的物理化学性质和核磁共振波谱图对所得单体进行结构分析。结果显示,从山慈菇80%乙醇的提取物中分离得到15个化合物,分别鉴定为正三十二烷醇(Ⅰ)、麦角甾醇(Ⅱ)、5-羟甲基糖醛(Ⅲ)、羊毛甾(Ⅳ)、没食子酸(Ⅴ)、shancigusinⅠ(Ⅵ)、天麻素(Ⅶ)、1-(4-b-D-吡喃葡萄糖基氧基苄基)-4-甲基-2-异丁基苹果酸(Ⅷ)、1-(4-b-D-吡喃葡萄糖基氧基苄基)-4-乙基-2-异丁基苹果酸(Ⅸ)、5’-methoxyisolariciresinol-9’-O-β-D-xylopyranoside(Ⅹ)、山药素Ⅲ(Ⅺ)、β-谷甾醇(Ⅻ)、葛根素(ⅩⅢ)、芥子酸(ⅩⅣ)和木犀草素(ⅩⅤ),其中化合物Ⅱ、Ⅹ和ⅩⅢ为首次从杜鹃兰属中分离得到,化合物Ⅱ、Ⅹ、ⅩⅢ和ⅩⅣ为首次从该植物中分离得到。

To study the chemical constituents of Cremastra appendiculata.Using silica gel column chromatography,Sephadex LH-20,Semi preparative high performance liquid chromatography,crystallization,recrystallization and other techniques to separate and purify the 80%ethanol extract of Cremastra appendiculata,and according to the physical and chemical properties of the obtained monomer compounds and nuclear magnetic resonance spectrum data Structure analysis and identification of the obtained monomer compounds.The results showed that 15 compounds were isolated from the 80%ethanol extracted species of Rhododendron sylvestris,which were identified as,Dotriacontanol(Ⅰ)、Ergosterol(Ⅱ)、5-hydroxymethylfurfural(Ⅲ)、lanosterol(Ⅳ)、Gallic acid(Ⅴ)、shancigusinⅠ(Ⅵ)、Gastrodin(Ⅶ)、1-(4-b-Dglucopyranosyloxybenzyl)4-methyl-2-isobutylmalate(Ⅷ)、1-1-(4-b-D-glucopyranosyloxybenzyl)4-ethyl-2-isobutylmalate(Ⅸ)、5’-methoxyisolariciresinol-9’-O-β-D-xylopyranoside(Ⅹ)、(3-Hydroxy-5-methoxyphenyl)-2-(3-hydroxyphenyl)ethane(Ⅺ)、Sitosterol(Ⅻ)、Daidzeine(ⅩⅢ)、Sinapic Acid(ⅩⅣ)、Luteolin(ⅩⅤ).It could condude that compoundsⅡ,Ⅹ,ⅪⅢwere isolated from the genus Cremastra Lindl.for the first time,and compoundsⅡ,Ⅹ,ⅩⅢ,ⅩⅣwere isolated from this plant for the first time.