香叶天竺葵精油和罗勒精油的抗抑郁功效及其作用机制研究

Antidepressant effect and mechanism of the essential oil from Pelargonium graveolens and Ocimum basilicum L.

研究两种芳香类中草药植物精油的抗抑郁功效及其作用机制。本文采用鼠尾悬挂和强迫游泳两种试验构建“行为绝望抑郁”模型,并测定模型小鼠不动时间;以皮质酮(CORT)诱导PC12细胞损伤来构建细胞应激损伤模型,MTT法检测细胞活力;对香叶天竺葵和罗勒精油抗抑郁功效及其作用机制进行研究。结果发现,在两种“行为绝望抑郁”模型试验中,与正常对照组相比,两种精油的各实验剂量组(0.24、0.36、0.48 mL/kg)均能有效缩短抑郁模型小鼠不动时间。而在CORT应激损伤细胞模型试验中,模型组细胞存活率明显降低,细胞突触收缩。当给予两种精油后,细胞存活率均显著提高,细胞突触有所恢复。另外,研究发现PI3K/Akt和PKA的信号通路阻断剂能显著减弱香叶天竺葵和罗勒精油对抗皮质酮的保护作用。以上结果表明,香叶天竺葵和罗勒的精油具有一定的抗抑郁作用,其神经保护作用可能由PI3K/Akt和PKA信号通路介导。

To study the antidepressant effect and mechanism of the essential oil(EO)from Pelargonium graveolens and Ocimum basilicum L..In this study,the model of“behavioral despair and depression”was established by using mouse tail suspension test(TST)and forced swimming test(FST),and then the immobility time of the model mice was measured.PC12 cells were injured by corticosterone(CORT)to establish stress injury model,and MTT assay was used to detect cell viability.The antidepressant effect and mechanism of EO from P.graveolens and O.basilicum were studied.The results showed that in the two“behavioral despair and depression”model tests,compared with the normal control group,each experimental dose group(0.24,0.36,0.48 mL/kg)of the two EO could effectively shorten the immobility time of depression model mice.However,in CORT stress injury cell model test,the cell survival rate of the model group decreased obviously,and the cell synapses contracted.When two kinds of EO were given,the cell survival rate was significantly improved,and the synapses were recovered.In addition,it was found that PI3K/Akt and PKA signal pathway inhibitor could significantly reduce the protective effect of geranium and basil essential oil against corticosterone.The above results indicated that EO have certain antidepressant effects,and their neuroprotective effects may be mediated by PI3K/Akt and PKA signaling pathways.