PRRS嵌合病毒活疫苗(PC株)和灭活疫苗联合免疫母猪应用试验

Application Test of Combined Vaccination of Live Chimeric Virus Vaccine(PRRSV PC Strain)and Inactivated Vaccine in Sows

为验证猪繁殖与呼吸综合征(PRRS)嵌合病毒活疫苗(PC株)和灭活疫苗联合应用于母猪的免疫效果,在天津市某PRRS阳性猪场,对部分母猪实施了两种疫苗联合免疫试验,利用RT-PCR和间接ELISA方法进行病毒和免疫抗体检测。结果显示:接种疫苗后,试验母猪精神、食欲和体温均正常,猪肛拭子和饲养环境样品中均未检测到病毒;免疫后14~70 d,试验母猪PRRS抗体阳性率均为100%;免疫后42 d,试验母猪的抗体IRPC平均值仍维持在较高水平,且免疫后14 d,2个试验组变异系数分别下降31和44个百分点;试验母猪所产仔猪的均匀度、活泼程度均高于对照组。结果表明,PRRS嵌合病毒活疫苗(PC株)和灭活苗联合免疫安全有效,不仅可以很好地诱导机体产生正常的免疫反应,而且还可以提高猪群抗体的整齐度且无排毒风险,免疫保护期较长。本研究为阳性猪场的PRRS防控提供了有效免疫方案。

In order to verify the immune eff ect of combined vaccination of chimeric virus vaccine(PC strain)and inactivated vaccine of porcine reproductive and respiratory syndrome(PRRS)applied in sows,some sows in a PRRS positive farm in Tianjin City were simultaneously vaccinated with the two kinds of vaccines,and related samples were collected and detected for virus nucleic acids and antibodies by RT-PCR and indirect ELISA,respectively.The results showed that the experimental sows were with normal spirit,appetite and body temperature,and no virus was detected in their samples of anal swabs and feeding environment;the positive rate was 100%in 14 to 70 days after vaccination;the average IRPC of antibodies remained at a relatively high level in 42 days after vaccination,and the variation coefficient of the two experimental groups decreased by 31%and 44%respectively in 14 days after vaccination;the evenness and liveliness of piglets produced by the experimental sows were higher than those by control group.In conclusion,combined vaccination of chimeric virus vaccine(PC strain)and inactivated vaccine of PRRS was safe and effective,which not only induced normal immune response in the body,but also improved the uniformity of antibodies in pigs without any risk of detoxifi cation,and provided a long immune protection period.An alternative and eff ective choice for PRRS prevention and control was thereby provided for PRRS-positive farms.