体外法研究亚麻籽油对瘤胃甲烷、氢气产量和脂肪酸组成的影响

Effects of Linseed Oil on Rumen Methane,Hydrogen Production and Fatty Acid Composition Using in Vitro Method

本试验从瘤胃氢代谢的角度出发,研究亚麻籽油抑制瘤胃甲烷生成的机制。试验采用单因子试验设计,采用全自动体外模拟瘤胃发酵设备,评估亚麻籽油添加剂量[0(对照)、1%、2%、3%、4%和5%]对瘤胃发酵48 h后产气量、氢气和甲烷产量及脂肪酸摩尔比例等的影响。选用3只装有永久性瘤胃瘘管的湘东黑山羊作为瘤胃液供体,每组2个平行,重复体外发酵试验3次。结果表明:与对照相比,随着亚麻籽油添加剂量的增加,产气量、干物质降解率、甲烷和氢气产量及肉豆蔻酸、棕榈酸、棕榈油酸、十七烷酸和不饱和脂肪酸摩尔比例线性降低,顺式油酸、亚麻酸和总不饱和脂肪酸摩尔比例线性升高,其中4%和5%的亚麻籽油作用效果达到显著水平(P<0.05)。综上所述,亚麻籽油中不饱和脂肪酸会通过生物氢化作用促进氢的利用,进而减少氢气产量,同时降低饲料降解和抑制甲烷生成。

In this study,the mechanism of linseed oil on rumen methane production was studied from the perspective of rumen hydrogen metabolism.The single factor experimental design was used to evaluate the effects of supplemental levels of linseed oil[0(control),1%,2%,3%,4%and 5%]on gas production,hydrogen and methane production and molar ratio of fatty acids after 48 h of rumen fermentation using the automatic fermentation incubation system.Rumen fluid was collected from three Xiangdong black goats with permanent rumen fistulas.Each group had 2 replicates,and were replicated 3 times.The results showed that the gas production,dry matter degradation,methane and hydrogen production,molar ratios of myristic acid,palmitic acid,palmitic oleic acid,heptadecanoic acid and saturated fatty acids in linseed oil groups were linearly decreased while the molar ratios of cis-oleic acid,linolenic acid and unsaturated fatty acids were linearly increased with the increasing of supplemental levels of linseed oil,and the effects of 4%and 5%linseed oil were significant(P<0.05).The unsaturated fatty acids in linseed oil enhance the hydrogen utilization,leading to decrease hydrogen production,meanwhile decrease the feed degradation and methane production.