胶原-乙酸混合溶液静电纺丝可纺性及电纺胶原膜力学特性评估

Spinnability of Collagen⁃acetic Acid Mixing Solution and Mechanical Properties of Electrospinning Nanofiber Membrane Evaluation

胶原蛋白具有可吸收降解、生物相容性好等特性,被广泛应用于生物医学领域。但是目前胶原蛋白静电纺丝胶原膜力学强度不高,限制了其在生物医学领域中的应用。本研究利用Ⅰ型胶原海绵与不同浓度乙酸混合,研究了乙酸胶原混合溶液的可纺性,并对制备的电纺胶原膜进行了力学性能评估。结果发现,质量比为40%乙酸与胶原配制成10%的乙酸-胶原混合溶液制备的胶原膜表面平整光滑,纤维平均直径为(305.19±119.67)nm,纤维与纤维间层次清晰、呈网状交错分布;厚度为0.2 mm的电纺胶原膜弹性模量为(749.22±107.72)MPa,最大拉伸力平均值为(21.92±3.03)N,具有较强机械性能。本研究利用乙酸-胶原混合溶液进行静电纺丝,获得了具有良好的外观、机械强度好的纳米胶原膜,为进一步的细胞学实验提供了材料,并有望开发成可满足不同临床需求的补片材料,具有一定的应用价值。

Collagen have good absorbability and biocompatibility, thus it is widely used in biomedicine. However, the application of collagen nanofiber membrane from electrospinning technique is restricted in biomedical field, for its lower mechanical strength. In this study, the spinnability of type I collagen mixing solution with acetic acid was studied, and the mechanical properties of collagen nanofiber membrane were measured. The results showed that the collagen nanofiber membranes obtained by 10% mixed solution using 40% acetic acid were smooth. The average diameter of the fibers was(305.19±119.67) nm, and the layers of fibers were clear and created a continuous crossed net. The elastic modulus of the collagen nanofiber membrane of 0.2 mm was(749.22±107.72) MPa, and the average maximum tensile strength was(21.92±3.03) N. This indicated that collagen nanofiber membrane of 0.2 mm had good mechanical properties. The collagen nanofiber membrane from mixed solution of acetic acid/collagen has good appearance and mechanical strength. It can provide materials for further cell experiments, and be developed into medical patch materials, which can meet different clinical needs and have the value of practical application.