响应面优化提取向日葵列当中苯乙醇苷的工艺研究

Optimization of Extraction Process of Phenylethanoid Glycosides from Orobanche cumana Wallr.Using Response Surface Methodology

以向日葵列当全草为原料,采用响应面优化乙醇加热回流法从向日葵列当中提取苯乙醇苷。以苯乙醇苷得率为评价指标,通过单因素试验设计,考察了溶剂质量分数、提取温度、提取时间、液固比和提取次数对苯乙醇苷提取效果的影响,并选用Central Composite Design响应面设计法分析建立了溶剂质量分数、提取温度、提取时间和液固比的二次多项式回归模型,优化提取工艺。结果表明,乙醇加热回流法提取向日葵列当中苯乙醇苷的最佳工艺参数为:溶剂质量分数为55%,提取温度为85℃,提取时间为90min,液固比为301(mL/g),提取4次。在此条件下,苯乙醇苷得率可达到10.223%,与模型预测值10.117%相当,建立的模型真实可靠。该提取方法简单高效,有利于向日葵列当资源的开发利用。

Response surface methodology was used to optimize the extraction conditions of phenylethanoid glycosides(PhGs)from Orobanche cumana Wallr by ethanol heating reflux method.Taking the yield of phenylethanoside as the evaluation index,the effects of solvent mass fraction,extraction temperature,extraction time,liquid solid ratio and extraction times on the extraction effect of PhGs were investigated through single factor experimental design.The quadratic polynomial regression model of solvent mass fraction,extraction temperature,extraction time and liquid solid ratio was established by using central composite design response surface design method,optimize the extraction process.The results showed the optimum technological parameters:the mass fraction of solvent 55%,the extraction temperature 85℃,the extraction time 90 min,the liquid-solid ratio 30 mL/g and four times.Under these conditions,the yield of phenylethanol glycoside can reach 10.223%,which was equivalent to the predicted value of 10.117%,and the established model was true and reliable.The extraction method is simple and efficient,which is conducive to the development and utilization of Orobanche cumana Wallr resources.