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蟾毒灵抑制肝癌干细胞lncRNAs分子筛选研究 被引量:3

Study on Screening of lncRNAs Involved in the Inhibitory Effect of Bufalin on Hepatocellular Carcinoma Stem Cells
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摘要 目的观察蟾毒灵对肝癌干细胞的影响,并探讨其相关机制。方法利用无血清悬浮培养技术富集肝癌干细胞球,分子生物实验检测肿瘤干细胞标记物CD133、CD44和上皮特异性抗原(ESA)表达,测序技术筛选蟾毒灵干预肝癌干细胞后lncRNAs分子的差异表达。结果通过无血清悬浮培养方法成功富集肝癌干细胞球,进一步发现蟾毒灵可抑制肝癌干细胞球的形成;蟾毒灵能显著抑制肝癌干细胞标记物CD133、CD44和ESA表达;测序结果显示,蟾毒灵可引起肝癌干细胞lncRNAs分子的差异表达。结论蟾毒灵具有抑制肝癌干细胞的作用,其机制可能与调控lncRNAs分子有关。 Objective To investigate the effects of bufalin on hepatocellular carcinoma stem cells;To discuss the possible mechanism.Methods Hepatocellular carcinoma stem cells spheres were enriched by serum-free suspension culture.Molecular biological experiments were used to detect the expressions of tumor stem cell markers CD133,CD44 and ESA in vitro,and sequencing technique was used to screen the differential expression of lncRNAs in hepatocellular carcinoma cells treated with bufalin.Results Hepatocellular carcinoma stem cells spheres were successfully enriched by serum-free suspension culture.Bufalin inhibited the formation of hepatocellular carcinoma cells spheres,and the expression of hepatocellular carcinoma stem cells markers CD133,CD44 and ESA were inhibited by bufalin in vitro.Sequencing result showed that bufalin induced the differential expression of lncRNAs.Conclusion Bufalin can inhibit hepatocellular carcinoma stem cells,and the mechanism may be related to the regulation of lncRNAs.
作者 王海永 张晨月 李佳 部帅 李成 赵成龙 WANG Haiyong;ZHANG Chenyue;LI Jia;BU Shuai;LI Cheng;ZHAO Chenglong(Shandong Cancer Hospital and Institute,Jinan 250117,China;Fudan University Shanghai Cancer Center,Shanghai 200032,China;Cancer Hospital Affiliated to Zhengzhou University,Zhengzhou 450003,China;The Second Affiliated Hospital of Shandong University of Traditional Chinese Medicine,Jinan 250001,China)
出处 《中国中医药信息杂志》 CAS CSCD 2021年第12期41-44,共4页 Chinese Journal of Information on Traditional Chinese Medicine
基金 山东省重点研发计划(2018GSF119014) 山东省泰山学者基金(tsqn201812149) 山东第一医科大学学术提升计划(2019RC004)。
关键词 蟾毒灵 肝癌干细胞 长链非编码RNA bufalin hepatocellular carcinoma stem cells lncRNAs
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