基于AHP-熵权法的D-最优设计响应面法优化云香喷剂提取工艺

Optimization of Extraction Process of Yunxiang Spray Based on AHP-Entropy Weight Method and D-Optimal Design Response Surface Method

目的采用D-最优设计响应面法优化云香喷剂提取工艺。方法在单因素试验基础上,以浸泡时间、加水倍数和提取时间为考察因素,以甘草酸、橙皮苷、厚朴酚、和厚朴酚含量及挥发油得率、干膏得率的AHP-熵权法综合评分为评价指标,进行多元线性回归及二项式拟合,绘制响应面图,选择最佳提取工艺并进行验证试验。结果最佳提取工艺为浸泡60 min、加13倍水、提取5.5 h;验证试验3批样品的综合评分分别为70.97%、69.15%、69.45%,RSD=1.40%。结论本研究优化的云香喷剂提取工艺简单可行,符合大生产要求,可为规范云香喷剂的提取工艺提供参考。

Objective To optimize the extraction process of Yunxiang Spray by D-optimal design response surface method.Methods On the basis of single factor experiment,soaking time,water ratio and extraction time as factors,and analytic hierarchy process(AHP)-entropy weight method comprehensive score of glycyrrhizic acid,hesperidin,magnolol,and honokiol,essential oil yield and the dry paste yield as evaluation indexes,multiple linear regression and the binomial fitting were conducted,and the response surface graph was drawn,in order to choose the optimal extraction process and conduct verification tests.Results The optimal extraction conditions were as follows:soaking for 60 min,adding 13 times water and extracting for 5.5 h.In the validation test,the comprehensive scores of the three batches of samples after extraction were 70.97%,69.15% and 69.45%,respectively,and the RSD was 1.40%.Conclusion The optimized extraction process in this study is simple and feasible,and meets the requirements of large-scale production,which can provide reference for the standardization of the extraction process of Yunxiang Spray.