自然状态下油松感染松材线虫后的生理响应

Physiological Response of Pinus tabulaeformis Infected with Bursaphelenchus xylophilus in Natural State

为研究自然状态下油松感染松材线虫后的生理响应,2019年10月在沈阳市东陵公园松材线虫发病区对受害前期、受害后期和健康对照组油松的1a生针叶进行取样,测定其抗氧化酶活性、渗透调节物质以及内源激素含量等指标。结果表明:受害前期过氧化物酶(POD)活性较对照组无显著性差异,受害后期较对照组和受害前期差异均极显著(p<0.01);受害前期超氧化物歧化酶(SOD)活性较对照组差异显著(p<0.05),受害后期较对照组和受害前期均无显著性差异;受害前期过氧化氢酶(CAT)活性较对照组差异极显著(p<0.01),受害后期较对照组和受害前期均无显著性差异;受害前期和受害后期脂氧合酶(LOX)活性较对照组均无显著性差异,但受害后期较受害前期差异性显著(p<0.05);受害前期苯丙氨酸解氨酶(PAL)活性较对照组无显著性差异,受害后期较对照组差异极显著(p<0.01),且较受害前期差异显著(p<0.05);受害前期丙二醛(MDA)含量较对照组差异极显著(p<0.01),受害后期较对照组无显著性差异,但较受害前期差异显著(p<0.05);受害前期可溶性蛋白(Spr)含量较对照组和受害后期均无显著性差异,受害后期较对照组差异性显著(p<0.05);类黄酮含量受害前期较对照组差异性显著(p<0.05),受害后期较对照组无显著性差异,但较受害前期差异性极显著(p<0.01);甜菜碱含量受害前期较对照组差异性显著(p<0.05),受害后期较对照组和受害前期均无显著性差异;水杨酸(SA)含量在各时期都差异极显著(p<0.01);受害前期茉莉酸(JA)含量较对照组和受害后期均无显著性差异,受害后期较对照组差异显著(p<0.05)。松材线虫会抑制油松SOD、CAT、LOX等抗氧化酶活性的表达,产生对细胞有害的物质MDA等。油松自身也通过增加可溶性蛋白、类黄酮、甜菜碱等物质的含量抵御松材线虫的侵染。内源激素SA、JA持续性增加,协调发挥作用,共同激发油松的抗逆反应。研究结果可为进一步研究油松对松材线虫的抗病机理奠定基础。

In order to study the physiological response of Pinus tabulaeformis infected with Bursaphelenchus xylophilus in natural state,the 1-year-old needles of Pinus tabulaeformis were sampled and analyzed in Dongling Park,Shenyang in October 2019.The activities of antioxidant enzymes,osmotic adjustment substances and endogenous hormone contents were determined.The results showed that the activity of peroxidase(POD)in the early stage of injury was not significantly different from that in the control group,but there was no significant difference in the late stage of injury compared with that in the control group and the early stage of injury(p<0.01);the activity of superoxide dismutase(SOD)in the early stage of injury was significantly different from that in the control group(p<0.05),but there was no significant difference in the late stage of injury compared with that in the control group and the early stage of injury;the activity of catalase(CAT)in the early stage of injury was significantly different from that in the control group(p<0.01),but there was no significant difference in the late stage of injury compared with that in the control group and the early stage of injury;the activity of lipoxygenase(LOX)in the early and late stages of injury was not significantly different from that in the control group,but there was significant difference in the late stage compared with that in the early stage of injury(p<0.05);the activity of phenylalanine ammonia lyase(PAL)in the early stage of injury was not significantly different from that in the control group,but there was significant difference in the late stage compared with that in the control group(p<0.01)and the early stage of injury(p<0.05);The content of malondialdehyde(MDA)in the early stage of injury was significantly different from that in the control group(p<0.01)and the late stage of injury(p<0.05),but there was no significant difference in the late stage of injury compared with that in the control group;the content of soluble protein(Spr)in the early stage of injury was not significantly different from that in the control group and the late stage of injury,but there was significant difference in the late stage compared with that in the control group(p<0.05);the content of flavonoid in the early stage of injury was significantly different from that in the control group(p<0.05)and the late stage of injury(p<0.01),but there was no significant difference in the late stage of injury compared with that in the control group;the content of betaine in the early stage of injury was significantly different from that in the control group(p<0.05),but there was no significant difference in the late stage of injury compared with that in the control group and the early stage of injury;the contents of salicylic acid(SA)were significantly different in each period(p<0.01);the jasmonic acid(JA)content in the early stage of injury was no significantly different from that in the control group and the late stage of injury(p>0.05),but there was no significant difference in the late stage of injury compared with that in the control group.These results indicate that the invasion of pine wood nematode can inhibit the expression of SOD,CAT,LOX and other antioxidant enzymes,and produce MDA which is harmful to cells.Pinus tabulaeformis could resist the infection of Bursaphelenchus xylophilus by increasing the content of soluble protein,flavonoids,betaine and other substances.The endogenous hormones SA and JA increased continuously and played a coordinated role to stimulate the stress resistance of Pinus tabulaeformis.This study can lay a foundation for further study on the resistance mechanism of Pinus tabulaeformis to Bursaphelenchus xylophilus.