DEPDC1B在膀胱癌组织中的表达及其临床相关性研究

Expression of DEPDC1B in bladder cancer and its clinical correlation

目的探讨DEPDC1B在膀胱癌组织中的表达及其临床相关性。方法选取2015年1月至2020年12月广东省水电医院泌尿外科收治的153例膀胱癌患者作为研究对象。采集其膀胱癌组织和癌旁组织,使用qRT-PCR方法检测组织中DEPDC1B、Rac1和ERK1/2的mRNA水平,使用Western Blot方法检测组织中DEPDC1B的蛋白水平,进而行统计学分析。结果DEPDC1B在膀胱癌组织中的mRNA水平高于癌旁组织,差异有统计学意义(P<0.05)。DEPDC1B在膀胱癌组织中的蛋白表达水平高于癌旁组织,差异有统计学意义(P<0.05)。DEPDC1B高表达组的膀胱癌分化程度和TNM分级高于DEPDC1B低表达组,差异有统计学意义(P<0.05)。DEPDC1B表达与膀胱癌患者的年龄、性别、肿瘤大小和数目无相关性(P>0.05)。膀胱癌组织中Rac1和ERK1/2的mRNA水平高于癌旁组织,差异有统计学意义(P<0.05)。结论DEPDC1B在膀胱癌中高表达,且与膀胱癌进展密切相关,DEPDC1B可能通过激活Rac1和ERK1/2发挥促癌作用。

Objective To explore the expression of DEPDC1B in bladder cancer and its clinical relevance.Methods A total of 153 cases of bladder cancer from January 2015 to December 2020 in the oncology department of our hospital were randomly selected as the research objects.The mRNA levels of DEPDC1B,NF-κB and ERK1/2 were detected by qRT-PCR,and the protein levels of depdc1b were detected by Western Blot.Results The level of mRNR of DEPDC1B in the cystic cancer tissue was higher than that in the paracellular tissue of carcinoma,with statistical significant difference(P<0.05).The protein expression level of DEPDC1B in the cystic cancer tissue was higher than that in the paracellular tissue of carcinoma,with statistical significant difference(P<0.05).The degree of differentiation and TNM grade of bladder cancer of the DEPDC1B high-expression group were higher than those of the DEPDC1B low-expression group,with statistical significant differences(P<0.05).There were no correlation between DEPDC1B expression with age,sex,tumor size,and number of patients with bladder cancer(P>0.05).The mRNA levels of Rac1 and ERK1/2 in the bladder cancer tissues were higher than those in the paracellular tissue of carcinoma,with statistical significant differences(P<0.05).Conclusion DEPDC1B is highly expressed in bladder cancer and is closely related to the progression of bladder cancer.DEPDC1B may play a role in promoting cancer by activating Rac1 and ERK1/2.