胆胃方对十二指肠食管反流模型大鼠蛋白酶激活受体-2及E-钙黏蛋白的影响

Effect of Danwei Formula on protease activated receptor-2 and E-cadherin in model rats with duodenal esophageal reflux

目的观察胆胃方对十二指肠食管反流模型大鼠蛋白酶激活受体-2(PAR-2)及E-钙黏蛋白的影响,探讨胆胃方治疗十二指肠食管反流的作用机制。方法选取72只SD大鼠,根据随机数字表法将其分为正常组、模型组、西药组和胆胃方低、中、高剂量组,各12只,采用“贲门缝合+食管十二指肠吻合术”制备十二指肠食管反流模型。胆胃方低、中、高剂量组分别予胆胃方1.46、2.92、5.84 g/ml,西药组予枸橼酸莫沙比利、铝碳酸镁混合溶液,模型组予生理盐水灌胃,按1 ml/100 g剂量灌胃,每日灌胃1次,共4周。观察食管下段黏膜形态及病理变化,免疫组化测定PAR-2及E-钙黏蛋白表达。结果与正常组比较,模型组大鼠洛杉矶分级积分及病理分级积分升高(P<0.05);与模型组比较,西药组、胆胃方低剂量组大鼠洛杉矶分级积分及病理分级积分降低(P<0.05)。与正常组比较,模型组大鼠食管组织PAR-2、E-钙黏蛋白平均光密度值升高(P<0.05);与模型组比较,西药组、胆胃方低剂量组及胆胃方中剂量组大鼠食管组织PAR-2平均光密度值降低(P<0.05),西药组、胆胃方低剂量组大鼠食管组织E-钙黏蛋白平均光密度值降低(P<0.05);与胆胃方低剂量组比较,胆胃方中、高剂量组大鼠食管组织PAR-2、E-钙黏蛋白平均光密度值升高(P<0.05)。结论胆胃方能改善模型大鼠食管黏膜损害,对十二指肠食管反流具有一定治疗效果。

Objective To observe the effect of Danwei Formula on protease activated receptor-2(PAR-2)and E-cadherin in duodenal esophageal reflux model rats in order to explore the mechanism of Danwei Formula in the treatment of duodenal esophageal reflux.Methods Seventy-two SD rats were selected and divided into normal group,model group,western medicine group,Danwei Formula low dose group,Danwei Formula medium dose group and Danwei Formula high dose group according to random number table method,with 12 rats in each group.The model of duodenoesophageal reflux was established by“cardia suture+esophago duodenostomy”.Danwei Formula low dose group,Danwei Formula medium dose group and Danwei Formula high dose group was given 1.46,2.92,5.84 g/ml of Danwei Formula respectively.Western medicine group was given mixed solution of Mosapride Citrate and Magnesium Aluminate.Model group was gavaged with normal saline at the dose of 1 ml/100 g,once a day for four weeks.Morphological and pathological changes of lower esophageal mucosa were observed,and the expressions of PAR-2 and E-cadherin were measured by immunohistochemistry.Results Compared with normal group,Los Angeles grade score and pathological grade score of rats in model group were higher(P<0.05).Compared with model group,Los Angeles grade score and pathological grade score of rats in western medicine group and Danwei Formula low dose group decreased(P<0.05).Compared with normal group,average optical density of PAR-2 and E-cadherin in esophageal tissue of rats in model group increased(P<0.05).Compared with model group,average optical density of PAR-2 in esophageal tissue of rats in western medicine group,Danwei Formula low dose group and Danwei Formula medium dose group decreased(P<0.05).Compared with model group,average optical density of E-cadherin in esophageal tissue of rats in western medicine group and Danwei Formula low dose group decreased(P<0.05).Compared with Danwei Formula low dose group,average optical density of PAR-2 and E-cadherin in esophageal tissue of rats in Danwei Formula medium dose group and Danwei Formula high dose group increased(P<0.05).Conclusion Danwei Formula can improve the damage of esophageal mucosa in model rats,and has a certain therapeutic effect on duodenal esophageal reflux.