基于网络药理学探讨桃仁-红花治疗视网膜静脉阻塞的作用机制

Discussion on the mechanism of peach seed-safflower in the treatment of retinal vein occlusion based on network pharmacology

目的运用网络药理学探讨桃仁-红花治疗视网膜静脉阻塞(RVO)的作用机制。方法利用中药系统药理学数据库与分析平台,预测桃仁-红花的成分及靶点,使用GeneCards数据库预测RVO的靶点,通过Jvenn获得交集靶点。利用Metascape数据库对交集靶点进行GO分析和KEGG通路富集分析,使用AutoDock软件对成分及靶点进行分子对接验证。结果获得桃仁-红花35个成分,178个靶点,与853个RVO靶点取交集,获得96个交集靶点。GO分析分别获得246个生物过程,61个细胞成分,111个分子功能,包括凋亡信号通路,对氧化应激的反应等生物学过程,KEGG分析共获得150条通路,包括AGE-RAGE信号通路、IL-17信号通路、PI3K-Akt信号通路等。分子对接结果显示成分进入机体后,能与靶点稳定结合并发挥疗效。结论桃仁-红花治疗RVO具有多成分、多靶点、多通路的特点,推测具体机制可能与改善血流动力学、调节炎症因子的表达、调控氧自由基的生成等有关。

Objective To explore the mechanism of peach seed-safflower in the treatment of retinal vein occlusion(RVO)by network pharmacology.Methods The composition and target of peach seed-safflower were predicted by traditional Chinese medicine systems pharmacology database and analysis platform,the target of RVO was predicted by GeneCards database,and the intersection target was obtained by Jvenn.Metascape database was used for GO analysis and KEGG pathway enrichment analysis of intersection targets,and AutoDock software was used for molecular docking verification of components and targets.Results A total of 35 components and 178 targets of peach seed-safflower were obtained,and 96 intersection targets were obtained by intersection of peach seed-safflower with 853 RVO targets.GO analysis obtained 246 biological processes,61 cell components and 111 molecular functions,including apoptotic signaling pathways,response to oxidative stress and other biological processes.A total of 150 pathways were obtained by KEGG analysis,including AGE-RAGE signaling pathway,IL-17 signaling pathway,PI3K-Akt signaling pathway,etc.Molecular docking results showed that the compounds could stably combine with the target and exert therapeutic effect after entering the body.Conclusion The treatment of RVO by peach seed-safflower is characterized by multiple components,multiple targets and multiple pathways.It is speculated that the specific mechanism may be related to improving hemodynamics,regulating the expression of inflammatory factors and regulating the generation of oxygen free radicals.