摘要
目的探讨lncRNA RAB11B-AS1对胃癌细胞增殖、迁移、侵袭和顺铂敏感性的影响及分子机制。方法建立顺铂耐药胃癌细胞系(HGC-27/DDP),将其分为对照组、si-NC组和si-RAB11B-AS1组;四甲基偶氮唑盐比色法(MTT)检测细胞存活率;实时荧光定量PCR(RT-qPCR)检测RAB11B-AS1和miR-628-3p的表达;流式细胞仪检测细胞周期;Transwell小室法检测细胞迁移和侵袭;双荧光素酶报告实验检测RAB11B-AS1和miR-628-3p的靶向关系。结果0.2、0.4 mg/L顺铂处理后HGC-27细胞存活率显著降低(P<0.05),而0~0.4 mg/L的顺铂处理后HGC-27/DDP细胞存活率无统计学意义。HGC-27和HGC-27/DDP细胞中RAB11B-AS1表达水平显著升高(P<0.05),且HGC-27/DDP细胞中RAB11B-AS1表达水平显著高于HGC-27细胞(P<0.05)。lncRNA RAB11B-AS1干扰表达,G0-G1期细胞所占比例显著升高,S期细胞所占比例显著降低(P<0.05);细胞存活率显著降低,迁移、侵袭细胞数显著降低(P<0.05)。RAB11B-AS1靶向调控miR-628-3p。结论LncRNA RAB11B-AS1可通过上调miR-628-3p表达抑制胃癌细胞增殖、迁移、侵袭,并提高癌细胞对顺铂的敏感性。
Objective To explore the effect and molecular mechanism of lncRNA RAB11B-AS1 on gastric cancer cell proliferation,migration,invasion and cisplatin sensitivity.Methods The cisplatin-resistant gastric cancer cells(HGC-27/DDP)were divided into control group,si-NC group,and si-RAB11B-AS1 group;tetra-methylazolium salt colorimetric assay(MTT)was used to detect cell survival rate;real-time fluorescence quantitative PCR(RT-qPCR)was applied to detect the expressions of RAB11B-AS1 and miR-628-3p;flow cytometry was used to detect cell cycle;cell migration and invasion were examined by Transwell cell method and dual luciferase report experiment was applied to detect the targeting relationship between RAB11B-AS1 and miR-628-3p.Results The survival rate of HGC-27 cells was significantly reduced after 0.2 and 0.4 mg/L cisplatin treatment(P<0.05),but the survival rate of HGC-27/DDP cells after 0-0.4 mg/L cisplatin treatment was not statistically changed.The expression of RAB11B-AS1 in HGC-27 andHGC-27/DDP cells was significantly increased(P<0.05),and the expression of RAB11B-AS1 in HGC-27/DDP cells was significantly higher than that of HGC-27 cells(P<0.05).Interfering with the expression of lncRNA RAB11B-AS1,the proportion of G0-G1 phase cells was increased significantly,the proportion of S phase cells was decreased significantly(P<0.05).Both cell survival rate and the number of migrating and invasive cells were significantly reduced(P<0.05).RAB11B-AS1 was found to specifically regulate miR-628-3p.Conclusions LncRNA RAB11B-AS1 can inhibit the proliferation,migration and invasion of gastric cancer cells by up-regulating the expression of miR-628-3p,and increase the sensitivity of cancer cells to cisplatin.
作者
杨宁波
贾晓东
寇耀
YANG Ning-bo;JIA Xiao-dong;KOU Yao(Department of Gastrointestinal Surgery, Suining Central Hospital, Suining 629000, China)
出处
《基础医学与临床》
2021年第12期1762-1766,共5页
Basic and Clinical Medicine
基金
四川省卫生厅科研计划项目(130493)。
