石蒜碱抑制胃癌细胞系MGC-803增殖、迁移和侵袭

Lycorine hydrochloride inhibits the proliferation,migration and invasion of gastric cancer cell line MGC-803

目的探究石蒜碱(LH)对人胃癌细胞系MGC-803细胞增殖、迁移、侵袭的影响及其可能的作用机制。方法MTT法和集落形成实验检测细胞增殖能力;流式细胞测量术检测细胞周期分布;划痕实验检测细胞迁移能力;Transwell小室法检测细胞侵袭能力;RT-qPCR检测迁移及侵袭相关基因表达。结果LH对MGC-803细胞活性的抑制呈浓度和时间双重依赖(P<0.05,P<0.01),48 h的IC_(50)值为3.76μmol/L;LH能显著抑制MGC-803细胞的集落形成,在浓度达到20μmol/L时集落形成受到完全抑制(P<0.01);LH作用后,将细胞周期阻滞在S期(P<0.01);随着LH浓度的增加,细胞迁移和侵袭受到明显的抑制(P<0.01);LH作用后细胞内miR-203基因表达水平上调,而Slug、MMP2、MMP9表达水平下调,并呈浓度依赖性(P<0.05,P<0.01)。结论LH明显抑制MGC-803细胞的增殖,诱导细胞周期阻滞于S期,抑制细胞的迁移和侵袭,上调细胞内miR-203基因表达水平。

Objective To investigate the effect of lycorine hydrochloride(LH)on the proliferation,migration and invasion of human gastric cancer cell line MGC-803 and its possible mechanism.Methods Cell proliferation ability was detected by MTT assay and colony formation assay.Distribution of cell cycle was detected by flow cytometry.The migration ability of cells was detected by wound healing assay.Transwell assay was used to detect the invasion ability of cells.RT-qPCR was used to detect the effects of LH on gene expression of cell migration and invasion.Results The inhibitory rate of LH on MGC-803 cells was concentration and time dependent(P<0.05,P<0.01),and the IC_(50) value at 48 h was 3.76μmol/L.The colony formation of MGC-803 cells was significantly inhibited by LH,and the colony formation was completely inhibited when the concentration reached 20μmol/L(P<0.01).Cell cycle was arrested in S phase after LH treatment(P<0.01).With the increase of LH concentration,cell migration and invasion were significantly inhibited(P<0.01),the expression level of miR-203 gene was up-regulated,while the expression levels of Slug,MMP2 and MMP9 were down-regulated in a concentration-dependent manner(P<0.05,P<0.01).Conclusions LH significantly inhibited the proliferation of MGC-803 cells,induced cell cycle arresting in the S phase,and inhibited cell migration and invasion.The expression level of miR-203 gene was up-regulated.