Tween80/BmimPF6/醇/水离子液体微乳制备及其萃取茶蛋白的工艺优化

Preparation of Tween80/BmimPF6/Alcohol/Water Ionic Liquid Microemulsion and Optimization of Its Extraction Process for Tea Protein

制备并优化Tween80/BmimPF6/醇/水离子液体微乳,并利用超声波辅助该微乳萃取茶蛋白。通过伪三元相图的绘制,探究助表面活性剂对微乳区域的影响,同时利用单因素试验和响应面优化Tween80/BmimPF6/醇/水离子液体微乳萃取茶蛋白的制备工艺。结果表明,乙醇作为助表面活性剂时,形成的离子液体微乳区域面积最大。Tween80/BmimPF6/醇/水离子液体微乳萃取茶蛋白最优工艺条件为萃取时间60 min、超声功率300 W、温度45℃、离子液体BmimPF6添加量0.2 mg/mL,茶蛋白萃取率为59.41%,与传统微乳法提取茶蛋白相比,萃取率增加13.04%。使用新型离子液体微乳萃取茶蛋白,提高了茶蛋白的萃取率,对茶蛋白萃取的实际应用和扩大化生产具有重要意义。

Tween80/BmimPF6/Alcohol/Water ionic liquid microemulsion was prepared and optimized,and the tea protein was extracted by the microemulsion assisted with ultrasound.The effect of surfactant on the microemulsion region was investigated by drawing the pseudo-ternary phase diagram,and the extractional process of tea protein by Tween80/BmimPF6/Alcohol/Water ionic liquid microemulsion was optimized by single factor test and response surface methodology.The results showed that when ethanol was used as a surfactant,the area of ionic liquid microemulsion was the largest.The optimum extraction conditions of tea protein by Tween80/BmimPF6/Alcohol/Water ionic liquid microemulsion were as follows:extraction time 60 min,ultrasonic power 300 W,temperature 45℃,addition of BmimPF6 to ionic liquid 0.2 mg/mL,extraction rate of tea protein was 59.41%,compared with the traditional microemulsion method,the extraction rate of tea protein was increased by 13.04%.A new type of ionic liquid microemulsion was used to extract tea protein,and the extraction rate of tea protein was increased.