Synergistic effect of T80/B30 vesicles and T80/PN320 mixed micelles with Se/C on nasal mucosal immunity

Selenium doped carbon(Se/C),an easily fabricated material,was found to be bio-active and it can serve as an adjuvant to enhance the immune effect of Tween 80/Brij 30(T80/B30)vesicles and Tween 80/polymer cationic surfactant PN320(T80/PN320)mixed micelles.The synergistic effect of the combination of T80/B30 vesicles and T80/PN320 mixed micelles with Se/C on nasal mucosal immunity was studied in this work,which might provide theoretical basis for developing the related new adjuvant for nasal immunization of recombinant protein,peptide and split protein vaccine.Since both selenium and carbon were bio-compatible elements,Se/C might be safe for practical applications,and this was also reflected by the low hemolytic activity of the materials.This work not only reports an efficient protocol for adjuvant development,but also significantly expands the application scope of selenium chemistry.