子宫内膜癌组织中miR-3188和mTOR表达量与预后的相关性研究

Study on the Correlation between the Expression Levels of miR-3188 and mTOR in Endometrial Cancer and the Prognosis

目的探究子宫内膜癌(endometrial carcinoma,EC)组织中微小核糖核酸-3188(miR-3188)和雷帕霉素靶蛋白(mTOR)表达量与预后的相关性。方法前瞻性选取2016年1月至2018年4月在开滦总医院林西医院接受手术治疗的121例EC患者作为研究对象,并根据3年内是否复发将其分为复发组(n=32)和未复发组(n=89)。用实时荧光定量PCR法检测EC组织和癌旁组织中miR-3188表达量,用蛋白质免疫印迹法检测EC组织和癌旁组织中mTOR表达量。结果EC组织中miR-3188相对表达量低于癌旁组织(0.60±0.14 vs 1.46±0.14),EC组织中mTOR相对表达量高于癌旁组织(0.92±0.11比0.63±0.11),差异均有统计学意义(t=48.995,19.348,均P=0.000)。不同浸润深度、是否淋巴结转移、是否子宫外转移、不同分化程度及不同FIGO分期的miR-3188和mTOR相对表达量比较,差异均有统计学意义(t=2.400~8.611,均P<0.05)。未复发组的miR-3188相对表达量高于复发组(0.66±0.11 vs 0.45±0.08),mTOR相对表达量低于复发组(0.88±0.10 vs 1.02±0.06),差异均有统计学意义(t=11.220,9.229,均P=0.000)。COX回归分析结果显示分化程度高(HR=0.696,P=0.015)、miR-3188表达量高(HR=0.058,P=0.008)是EC预后的独立保护因素,FIGO分期高(HR=2.967,P=0.000)、mTOR表达量高(HR=1.162,P=0.035)是EC预后的独立危险因素。Pearson相关分析结果显示EC组织中miR-3188相对表达量与mTOR相对表达量呈负相关关系(r=-0.409,P=0.000)。结论EC组织中miR-3188表达量低、mTOR表达量高与预后不良有关。

Objective To explore the correlation between the expression of microRNA-3188(miR-3188),mammalian target of rapamycin(mTOR)in endometrial carcinoma(EC)and the prognosis.Methods A total of 121 patients with EC who received surgical treatment in Linxi Hospital of Kailuan General Hospital from January 2016 to April 2018 were prospectively selected and divided into relapsed group(n=32)and non-relapsed group(n=89)according to recurrence within 3 years.Real-time fluorescence quantitative PCR was used to detect the expression of miR-3188 in EC tissues and adjacent tissues,and Western blotting was used to detect the expression of mTOR in EC tissues and adjacent tissues.Results The relative expression of miR-3188 in EC tissue was lower than that in adjacent tissues(0.60±0.14 vs 1.46±0.14),and the relative expression of mTOR in EC tissue was higher than that in adjacent tissues(0.92±0.11 vs 0.63±0.11),the differences were statistically significant(t=48.995,19.348,all P=0.000).The relative expression levels of miR-3188 and mTOR were compared with different depth of invasion,lymph node metastasis,extrauterine metastasis,different degree of differentiation,and different FIGO stages,and the differences were statistically significant(t=2.400~8.611,all P<0.05).The relative expression of miR-3188 in the non-relapsed group was higher than that in the relapsed group(0.66±0.11 vs 0.45±0.08),and the relative expression of mTOR was lower than that in the relapsed group(0.88±0.10 vs 1.02±0.06),the differences were statistically significant(t=11.220,9.229,all P=0.000).COX regression analysis showed that high degree of differentiation(HR=0.696,P=0.015)and high expression of miR-3188(HR=0.058,P=0.008)were independent protective factors for the prognosis of EC,and high FIGO staging(HR=2.967,P=0.000)and high expression of mTOR(HR=1.162,P=0.035)were independent risk factors for the prognosis of EC.The results of Pearson correlation analysis showed that the relative expression of miR-3188 in EC tissues was negatively correlated with the relative expression of mTOR(r=-0.409,P=0.000).Conclusion Low expression of miR-3188 and high expression of mTOR in EC tissues are related to poor prognosis.