三阴性乳腺癌患者血清miR-146a,miR let-7a水平表达与临床病理特征和化疗疗效的相关性研究

Correlation of Serum miR-146a and miR let-7a Expression with Clinicopathological Characteristics and Chemotherapy Efficacy in Patients with Triple Negative Breast Cancer

目的探究三阴性乳腺癌(triple negative breast cancer, TNBC)患者血清miR-146a和miR let-7a水平表达与临床病理特征和化疗疗效的相关性。方法选择陕西中医药大学附属医院2014年7月~2016年6月行乳腺癌根治术并经免疫组化(immunohistochemical, IHC)检测证实为TNBC的79例患者为研究对象。采用实时荧光定量PCR(quantitative real-time PCR, qRT-PCR)检测血清miR-146a和miR let-7a的水平。相关性采用Spearman秩相关分析。结果血清miR-146a和miR let-7a在肿瘤直径≥3 cm,临床分期Ⅲ期和淋巴结转移的患者中的表达水平高于肿瘤直径<3 cm者,临床分期Ⅰ~Ⅱ期和无淋巴结转移的患者,差异具有统计学意义(t=7.41~10.39,均P <0.05)。TNBC患者化疗后血清miR-146a和miR let-7a表达水平(0.81±0.32和0.60±0.26)显著低于化疗前(1.77±0.51和1.92±0.77),差异具有统计学意义(t=11.31,10.11,均P=0.00)。疾病控制组患者血清miR-146a和miR let-7a表达水平(0.37±0.12和0.51±0.17)低于疾病进展组(1.40±0.79和1.62±0.55),差异具有统计学意义(t=8.97,10.22,均P <0.05)。Spearman秩相关分析显示血清miR-146a和miR let-7a表达水平与化疗疗效呈负相关(r=-0.199,-0.150,均P <0.01)。结论血清miR-146a和miR let-7a表达水平与肿瘤直径、临床分期、淋巴结转移和化疗疗效相关。

Objective To explore the correlation between serum miR-146 a and miR let-7 a expression and clinicopathological characteristics and chemotherapy efficacy in patients with triple negative breast cancer(TNBC). Methods Seventy-nine patients with TNBC who underwent radical mastectomy of breast cancer and were confirmed by immunohistochemical(IHC) detection in the Affiliated Hospital of Shaanxi University of traditional Chinese Medicine from July 2014 to June 2016 were selected as the research subjects. Quantitative real-time PCR(qRT-PCR) was used to detect the levels of serum miR-146 a and miR let-7 a.Spearman rank correlation analysis was used for correlation analysis. Results The expression level of serum miR-146 a and mi R let-7 a in patients with tumor diameter ≥ 3 cm, clinical stage Ⅲ and lymph node metastasis was higher than that in patients with tumor diameter<3 cm, clinical stage Ⅰ~Ⅱ and no lymph node metastasis, and the difference were statistically significant(t=7.41~10.39, all P<0.05). The expression levels of serum miR-146 a and miR let-7 a in TNBC patients after chemotherapy(0.81±0.32 and 0.60±0.26) were significantly lower than those before chemotherapy(1.77±0.51 and 1.92±0.77), and the difference was statistically significant(t=11.31, 10.11, all P=0.00). The expression levels of serum miR-146 a and miR let-7 a in the disease control group(0.37±0.12 and 0.51±0.17) were lower than those in the disease progression group(1.40±0.79 and 1.62±0.55), and the difference was statistically significant(t=8.97, 10.22, all P<0.05). Spearman rank correlation analysis showed that the expression levels of serum miR-146 a and miR let-7 a were negatively correlated with chemotherapy efficacy(r=-0.199,-0.150, all P<0.01). Conclusion The expression levels of serum miR-146 a and miR let-7 a were correlated with tumor diameter,clinical stage, lymph node metastasis and chemotherapy efficacy.