水稻NRL基因家族的全基因组鉴定与生物信息学分析

Genome-wide Identification and Bioinformatics Analysis of NRL Gene Family in Rice

NRL (NPH3/RPT2-Like)基因家族是植物中特异存在的基因家族,根据拟南芥NRL基因家族中参与植物向光性响应的NPH3 (NONPHOTOTROPIC HYPOCOTYL 3)和RPT2 (ROOT PHOTOTROPISM 2)命名。本研究利用生物信息学方法鉴定到27个水稻NRL基因,分布在除10号染色体外的染色体上,同时基因复制分析结果表明,全基因复制或片段复制比串联复制在水稻NRL基因家族扩张中起更大的作用。利用单、双子叶植物的7个物种进行系统进化关系分析,将192个NRL基因家族成员分为6个不同的组。水稻NRL家族的结构特征分析显示,多数成员含有NRL基因家族的特征结构域,而基于RNA-Seq和微阵列数据的表达分析结果表明,水稻NRL家族基因在多个组织表达,同时部分基因在叶片、花序和花药中高表达。本研究对水稻NRL基因家族进行鉴定和分析,为进一步研究水稻NRL基因家族的功能提供了一定的参考和依据。

The NRL(NPH3/RPT2-Like) gene family is plant-specific and it is named after its two members in Arabidopsis, NPH3(NONPHOTOTROPIC HYPOCOTYL 3) and RPT2(ROOT PHOTOTROPISM 2), which are involved in phototropism. The study identified 27 rice NRL genes distributed on 12 chromosomes except chromosome 10 with bioinformatic analysis. Gene duplication analysis revealed that whole genome duplication(WGD)/segmental duplication played a more vital role than tandem duplication in rice NRL gene family expansions. Based on phylogenetic analysis of 7 monocotyledons, 192 NRL gene family members were divided into 6 different groups.Structural feature analysis showed that most members of rice NRL gene family contained specific domains of NRL family. Furthermore, based on RNA-Seq data and microarray data, expression analysis showed that rice NRL family genes were expressed in several tissues and some genes displayed higher expression in leaves, inflorescence and anther. Identification and analysis were conducted in NRL gene family from rice and these results provided some reference-able messages for further functional research.