滇水金凤TTG1同源基因的克隆及表达分析

Cloning and Expression Analysis of TTG1 Homologous Genes in Impatiens uliginosa

TTG1 (TRANSPARENT TESTA GLABRA1)基因编码具有WD40重复序列的蛋白,是调控植物花青素合成的重要因子。本研究以滇水金凤(Impatiens uliginosa)花器官为材料,采用RT-PCR技术克隆得到滇水金凤TTG1同源基因,其cDNA全长为1 038 bp,编码345 aa,命名为IuTTG1。系统发育分析发现,IuTTG1与百脉根聚为一支,同源性达81.84%。通过荧光定量分析发现,IuTTG1在4种不同花色以及4个不同花发育时期的滇水金凤中均有表达,其中以深红色滇水金凤的盛花期表达量最高,白色滇水金凤的始花期表达量最低。本研究结果为进一步探究滇水金凤花色形成及变异机理、花色改良及新品种培育提供科学依据。

TTG1(TRANSPARENT TESTA GLABRA1) gene encodes a protein with WD40 repeat sequence,which plays an important role in regulating the biosynthesis of anthocyanin in the plants. In this study, the floral organs of Impatiens were used as experimental material, and the TTG1 homologous gene of Impatiens was obtained by useing RT-PCR method, whose c DNA sequence was 1 038 bp, encoded 345 amino acids, and named IuTTG1. Phylogenetic analysis showed that IuTTG1 is clustered with Lotus sessilifolius and the homology reached81.84%. According to RT-qPCR analysis, IuTTG1 gene was expressed in both four different colors of Impatiens and their four different stages of flower development, among which the highest expression level was in the blooming stage of deep-red flower and the lowest expression level was in the beginning flowering stage of whiteflower. The results of this study provide scientific basis for further exploring the formation and variation mechanism of the flower color of Impatiens, improving the flower color of Impatiens and cultivating new species.