基于ISSR分子标记技术的蓝花楹遗传多样性和亲缘关系分析

Analysis of Genetic Diversity and Relationship of Jacaranda mimosifolias Based on ISSR Molecular Markers Technique

利用ISSR分子标记技术对来自不同省份的19个种源共21份蓝花楹种质资源进行遗传多样性分析,探讨其亲缘关系。从33条ISSR引物中筛选出10条引物组合得到53个清晰的电泳条带,其中多态性条带44条,多态性百分率为83.02%,平均每条引物扩增出5.3个条带。21份蓝花楹种质观察等位基因数(Na)平均为1.83,有效等位基因总数(Ne)平均为1.45,Nei’s基因多样性指数(H)平均为0.27,Shannon’s信息指数(I)平均为0.40。ISSR分子标记聚类分析研究表明,21份蓝花楹种质资源可聚为两大类群,且具有丰富的遗传多样性,其亲缘关系与栽培区地理来源相关性不强。本研究较准确地进行了各蓝花楹种质资源的分子鉴别,可为蓝花楹的良种选育提供理论依据。

The genetic diversity and relationship of Jacaranda mimosifolias from 19 provenances including 21 germplasms in China were studied by ISSR molecular markers technique. 53 bands were amplified by using 10 selected primers from all the 33 primers, of which 44 bands were polymorphic, with the polymorphism ratio of 83.02%. Each primer could amplify 5.3 bands in average. The average number of tested alleles(Na), effective alleles(Ne), Nei’s gene diversity index(H) and Shannon’s information index(I) were 1.83, 1.45, 0.27, 0.40, respectively.ISSR molecular marker cluster analysis research shows 21 J. mimosifolias germplasm resources could be divided into two groups, and the genetic diversity is high. Their genetic relationship is not strongly related to geographical origin of cultivation area. In this study, the relatively accurately molecular identification of the germplasm resources of J. mimosifolias was carried out, which will provide a theoretical basis for the breeding of the improved varieties.