甘草甜素调节U14宫颈癌小鼠免疫功能及诱导凋亡的作用

Effect of Glycyrrhizin on Immune Function and Apoptosis of U14Cervical Cancer Mice

为探讨甘草甜素调节U14宫颈癌小鼠免疫功能及诱导凋亡作用,将U14宫颈癌小鼠随机分为模型组、甘草甜素低、中、高剂量组(25、50、100 mg/kg)及环磷酰胺组(25 mg/kg),另设正常组,每组10只;灌胃给药,1次/d,共21 d。测量各组小鼠瘤体积(最大直径)、瘤质量,计算胸腺指数、脾脏指数,TUNEL法检测细胞凋亡指数,实时定量PCR法检测Caspase-3、Bax及Bcl-2 mRNA表达,Western Blot法检测p-PI3K、p-AKT蛋白表达等指标。结果发现,与模型组比较,甘草甜素低、中、高剂量组小鼠瘤体积(最大直径)及瘤质量显著降低(P<0.05或P<0.01),抑瘤率分别为15.68%、25.41%和38.38%;与模型组比较,甘草甜素低、中、高剂量组小鼠胸腺指数、脾脏指数、IL-2、IFN-γ、TNF-α含量、凋亡指数、Caspase-3及Bax mRNA表达显著增加(P<0.05或P<0.01),而Bcl-2 mRNA及p-PI3K、p-AKT蛋白表达显著降低(P<0.05或P<0.01)。上述结果表明,甘草甜素具有抑制U14宫颈癌小鼠肿瘤生长的作用,该作用与改善免疫功能及诱导凋亡作用有关。

To investigate the effect of glycyrrhizin on regulating immune function and inducing apoptosis in U14 cervical cancer mice, U14 cervical cancer mice were randomly divided into model group, low-dose, medium-dose and high-dose glycyrrhizin groups(25, 50 and 100 mg/kg), cyclophosphamide group(25 mg·kg;) and normal group with 10 mice in each group. Intragastric administration was performed once a day for 21 days. Tumor volume(maximum diameter) and tumor mass were measured, thymus index and spleen index were calculated, apoptosis index was detected by TUNEL assay,mRNA expressions of Caspase-3, Bax and Bcl-2 were detected by real-time quantitative PCR, and protein expressions of pPI3 K and p-Akt were detected by Western Blot. The results showed that the tumor volume(maximum diameter) and tumor weight in low-dose, medium-dose and high-dose glycyrrhizin groups were significantly lower than those in the model group(P<0.05 or P<0.01) after 21 days of administration, and the tumor inhibition rates were 15.68%, 25.41% and 38.38%,respectively. Compared with the model group, thymus index, spleen index, IL-2, IFN-γ, TNF-α contents, apoptosis index,Caspase-3 and Bax mRNA expressions in low-dose, medium-dose and high-dose glycyrrhizin groups were increased(P<0.05 or P<0.01), while Bcl-2 mRNA and p-PI3 K and p-AKT protein expressions were decreased(P<0.05 or P<0.01).The above results indicate that glycyrrhizin can inhibit tumor growth in U14 cervical cancer mice, which is related to improving immune function and inducing apoptosis.