高通量测序研究清水池底泥微生物群落多样性

Study on Microbial Community Diversity in Sediment of Clean Water Tank by High-throughput Sequencing

采用Illumina MiSeq高通量测序技术研究了北方某水厂清水池底泥的微生物菌群结构,基于门、属水平剖析细菌、古细菌、真菌的种类和相对丰度,同时探讨了其产生的原因及对用户水质安全的影响,并提出了解决措施和建议。清水池底泥共检测到30门、55纲、79目、152科、410属细菌,9门18属古细菌,以及4门16属真菌;清水池底泥中的微生物菌群丰度较高,均属于水体和土壤中常见的微生物,有微量可能致病的菌属存在,供水水质存在安全隐患。分析原因,可能是消毒不完全以及管道存在腐蚀等所致。因此建议,适当增加消毒剂投量、保证管网中的消毒剂余量,加强对供水管网的维护、管理和水质检测。

The microbial community in the sediment of clean water tank from a waterworks in northern China was analyzed by Illumina MiSeq high-throughput sequencing.The species and relative abundances of bacteria,archaea and fungi were analyzed at phylum and genus levels.The causes of their growth and their influence on the safety of the user water quality were explored,and the solutions and suggestions were proposed.The bacterial community of the sediment included 30 phyla,55 classes,79 orders,152 families and 410 genera,the archaeal community had 9 phyla and 18 genera,and the fungal community consisted of 4 phyla and 16 genera.The abundance of the microbes in the sediment of the clean water tank was high,and they all belonged to the common microorganisms in water and soil.There were trace bacteria that might cause disease and endangered the security of the water quality.The reasons might be incomplete disinfection and pipeline corrosion.Therefore,it was suggested to appropriately increase the dosage of disinfectant,ensure the residual disinfectant in the pipe network,and strengthen the maintenance,management and water quality detection of the water supply pipe network.