摘要
A novel fluorescent composition was firstly isolated from natural winter fresh Moso bamboo shoots,and its optical properties were fully investigated by fluorescence spectroscopy.It could emit strong blue light both in solid and solution state,providing high fluorescence intensity in ethanol.The solution’s concentration and addition of water greatly affected the fluorescence intensity,high concentration and addition of much water could quench fluorescence.Apoptosis results showed that the fluorescent extract(0-25 mg/L)could not induce apoptosis of Hela cells.Confocal fluorescent microscopic imaging in human hepatocellular carcinoma cells(HepG2)was realized using the fluorescent extract,it could dye the whole cell well which was different from 4',6-diamidino-2-phenylindole(DAPI)only dying cell nucleus.The fluorescent extract may be candidate for future natural fluorescent bio-imaging agent.
基金
by the Science and Technology Planned Projects of Zhejiang Province(2018F10009)
the National Key Research&Development(R&D)plan(2016YFD060090305)
the Promotion of Project of Forestry Science and Technology of the Chinese Forestry Administration([2016]22)
the International Joint Project Between Mars Incorporated and China National Bamboo Research Center(PRSD-026-13)
the Science and Technology Planned Projects of Zhejiang Province(2017E8002).
