响应面优化超声辅助提取竹笋壳棕色素及其稳定性研究

Optimization of ultrasonic-assisted extraction and stability analysis of the brown pigment from bamboo shoot shell using response surface methodology

为实现废弃竹笋壳的资源化利用,采用超声辅助-水提法,研究了提取时间、超声功率、料液比以及提取次数对棕色素提取效果的影响,并通过响应面曲法对竹笋壳棕色素的提取工艺进行优化,同时研究了竹笋壳棕色素在不同条件下的稳定性。研究结果表明,最佳的提取时间为25.46min、超声功率为205.46W、料液比为1∶22.97g/mL、提取次数为1次,在最佳的工艺条件下,竹笋壳棕色素的提取效果最好(吸光度为1.302)。该工艺提取的竹笋壳棕色素对食品添加剂(蔗糖、NaCl)和大多数金属离子(K^(+)、Ca^(2+)、Mg^(2+)和Al^(3+))都表现出较强的稳定性,同时还具有较好的耐酸碱性和耐高温性,而氧化还原剂(H_(2)O_(2)、Na_(2)SO_(3))和光照(强烈太阳光、紫外光)对其稳定性存在一定的影响。

In order to utilize the waste bamboo shoot shells,the brown pigment extraction from shells was studied by ultrasonic-assisted water extraction.The effects of extraction time,ultrasonic power,solid-liquid ratio and extraction times on the yield of pigment were investigated.The extraction process was optimized by response surface methodology,the stability of the brown pigment under different conditions was compared as well.Results showed that the best extraction conditions were:extraction time 25.46 min,the ultrasonic power 205.46W,the solid-liquid ratio 1∶22.97g/mL and the extraction for one time.The absorbance of brown pigments was 1.302 under the above optimal extraction conditions.The brown pigment was very stable to food additives(sucrose and NaCl)and most of the metal ions(K^(+),Ca^(2+),Mg^(2+)and Al^(3+)),good resistance to acid and alkaline and high temperature.However,the redox agents(H_(2)O_(2),Na_(2)SO_(3))and light(strong sunlight,ultraviolet light)affected the pigment’s stability.