胰腺癌组织中SMC1A表达的临床意义及对细胞增殖的影响

The clinical significance of SMC1A expression in pancreatic cancer and its influence on cell proliferation

目的探讨胰腺癌细胞中染色体结构维持蛋白1A(SMC1A)表达的临床意义及其对细胞增殖的影响。方法选取2010年1月至2015年12月锦州医科大学附属第一医院肿瘤内科59例手术切除并经术后病理证实的胰腺癌标本,免疫组化检测SMC1A蛋白表达,采用χ2检验分析SMC1A蛋白表达与各临床指标的相关性。检测不同肿瘤来源胰腺癌细胞中SMC1A蛋白和m RNA表达及其对胰腺癌细胞增殖的影响。结果胰腺癌中SMC1A明显高表达,其表达与肿瘤大小(P=0.034)、T分期(P=0.003)及UICC分期(P=0.047)正相关。SMC1A高表达、UICC分期晚是影响预后的独立危险因素(均P<0.05)。原发胰腺癌细胞中SMC1A表达显著低于转移性胰腺癌细胞,且SMC1A干扰后胰腺癌细胞增殖被抑制。结论胰腺癌中SMC1A高表达,且表达与肿瘤大小、T分期、UICC分期密切相关。SMC1A高表达是影响预后的独立危险因素,其作用机制可能是SMC1A表达促进了胰腺癌细胞增殖。

Objective To investigate the clinical significance of the expression of chromosome structure maintenance protein 1 A(SMC1 A)in pancreatic cancer cells and its effect on cell proliferation.Methods From January 2010 to December 2015,59 specimens of pancreatic cancer patients who underwent surgical resection and who had pathologically confirmed postoperative pathology were selected from The First Affiliated Hospital of Jinzhou Medical University.Results SMC1 A was significantly expressed in pancreatic cancer,and its expression was positively correlated with the tumor size(P=0.034),T stage(P=0.003),and Union for International Cancer Control(UICC)stage(P=0.047).The positive and high expression of SMC1 A and the UICC stage were independent risk factors affecting the prognosis(all P<0.05).The expression of SMC1 A in primary pancreatic cancer cells was significantly lower than that in metastatic pancreatic cancer cells,and the proliferation of pancreatic cancer cells was inhibited after SMC1 A interference.Conclusion SMC1 A is highly expressed in pancreatic cancer,and its expression is closely related to the tumor size,T stage,and UICC stage.The high expression of SMC1 A is an independent risk factor affecting the prognosis,and its mechanism may be that SMC1 A expression promotes the proliferation of pancreatic cancer cells.