基于网络药理学探讨甘麦大枣汤抗抑郁的作用机制

Study on the Action Mechanism of Ganmai Dazao Decoction on Depression Based on Network Pharmacology

目的:研究基于网络药理学探讨甘麦大枣汤抗抑郁的作用机制。方法:应用中药系统药理学数据库与分析平台(TCMSP)、中药分子机制生物信息学分析工具库(BATMAN-TCM)及文献搜索获取甘草、小麦、大枣的活性成分及作用靶点,从GeneCards数据库获得抑郁症靶点,获得药物疾病交集靶点,通过String在线平台数据库构建蛋白质-蛋白质相互作用(PPI)网络,并利用Cytoscape软件进行可视化及拓扑学分析筛选核心基因,应用DAVID数据库完成基因本体(GO)功能与京都基因与基因组百科全书(KEGG)通路的富集分析。结果:共筛出中药活性成分94个,有效靶点49个,药物疾病交集靶点48个。PPI网络发现ALB、CASP3、VEGFA、MYC、CCND1、ESR1、CTNNB1、AR等可能是关键靶点。GO功能富集分析得到181个生物过程,主要涉及雌激素反应、胆碱能突触传递等。KEGG通路富集分析发现46个通路,主要集中在癌症相关通路、甲状腺激素信号通路、PI3K/AKT信号通路、Wnt信号通路等。结论:本研究为甘麦大枣汤的药效物质基础的进一步研究提供了理论基础,同时为其在抗抑郁方面的应用提供了参考依据。

Objective:Based on network pharmacology,the action mechanism of Ganmai Dazao Decoction was investigated.Methods:The active ingredients and targets of Radix Glycyrrhizae,wheat and Fructus Jujubae were obtained by using the Traditional Chinese Medicine System Pharmacology Database and Analysis Platform(TCMSP),the Chinese Medicine Molecular Mechanism Bioinformatics Analysis Tool Library(BATMAN-TCM)and the literature search.From the GeneCards database we obtained depression targets and drug disease intersection targets,constructed a protein-protein interaction(PPI)network through the String online platform database,and used Cytoscape software for visualization and topological analysis to screen core genes,and used the DAVID database to complete the gene ontology(GO)function and enrichment analysis of Kyoto Encyclopedia of Genes and Genome(KEGG)pathway.Results:A total of 94 active ingredients of Chinese medicinal,49 effective targets,and 48 drug disease intersection targets were screened out.PPI network found that ALB,CASP3,VEGFA,MYC,CCND1,ESR1,CTNNB1,AR,etc.may be key targets.GO functional enrichment analysis revealed 181 biological processes,mainly involving estrogen response and cholinergic synaptic transmission.KEGG pathway enrichment analysis found 46 pathways,mainly focusing on cancer-related pathways,thyroid hormone signaling pathway,PI3K-AKT signaling pathway,Wnt signaling pathway,etc.Conclusion:This study provides a theoretical basis for further research on the effective material basis of Ganmai Dazao Decoction,and at the same time provides a reference basis for its application in antidepressant.