Sdr9c7基因在荷瘤小鼠红细胞中异常表达的研究

Study on the Abnormal Expression of Sdr9c7 Gene in Erythrocytes of Tumor-bearing Mice

与正常小鼠外周血的红细胞不同,Lewis癌细胞(Lewis lung carcinoma,LLC)的荷瘤雄性小鼠的红细胞在外周血白细胞分离实验中并没有沉底,而是处于中间层。为探讨Sdr9c7基因的功能及荷瘤状态下红细胞发生异常的可能机制,采集实验小鼠的外周血,分离了红细胞、白细胞,并以Sdr9c7基因高表达的皮肤、肝脏、骨髓、血管作为对照组织,采用实时荧光定量PCR法检测Sdr9c7基因在正常小鼠和荷瘤小鼠各组织细胞中的表达变化情况。结果表明:相对于对照组,Sdr9c7基因唯独在荷瘤小鼠组的红细胞中表达显著上升,是正常小鼠红细胞的2.60±0.44倍;在皮肤、血管中表达无显著差异;在荷瘤小鼠组的血液、骨髓、肝脏、白细胞中的表达则显著下调,分别是正常小鼠相应组织细胞的0.31±0.05、0.44±0.04、0.36±0.04、0.35±0.08倍,表明荷瘤小鼠红细胞异常的发生可能与Sdr9c7基因表达上调有关。实验结果为进一步研究Sdr9c7基因的生物学功能拓宽视野,并为荷瘤状态下红细胞发生异常的机制提供新视角。

In the peripheral blood leukocyte separation experiment,the red blood cells of tumor-bearing male mice with Lewis cancer cell(Lewis lung carcinoma,LLC)did not sink to the bottom but were stagnated in the middle layer,which was different from that of normal mice.To explore the further function of the Sdr9c7 gene and the possible mechanism of erythrocyte abnormality in tumor-bearing male mice,the expression of the Sdr9c7 gene was detected by real-time fluorescence quantitative PCR in red blood cells and lymphocyte isolated from the peripheral blood,and some tissues such as skin,liver,bone marrow,and blood vessels.Compared with the control group,the expression level of the Sdr9c7 gene in erythrocytes of tumor-bearing mice increased significantly,which was 2.60±0.44 times as that of normal mice.Although its expression showed no significant difference in skin and blood vessels,its expression in blood,bone marrow,liver,and lymphocyte of tumor-bearing mice were significantly down-regulated,which was 0.31±0.05,0.44±0.04,0.36±0.04 and 0.35±0.08 times as that of normal mice separately.The results suggested that the occurrence of erythrocyte abnormality in tumor-bearing mice might be related to the increased of Sdr9c7 gene expression.This result broadens the horizon for further study of the biological function of Sdr9c7 gene and provides a new perspective for the mechanism of erythrocyte abnormalities in the tumor-bearing state.