基于汞离子检测所构建的电致化学发光生物传感器中不同信号输出模式的最新研究进展

The latest research progress of different signal output modes in electrochemiluminescence biosensors based on mercury ion detection

汞离子(Hg^(2+))是最危险的重金属污染物之一,易从环境迁移到食物链中,在人体累积,对大脑、神经系统、内分泌系统、肾脏等造成严重损害。发展快速、灵敏和准确的定量检测Hg^(2+)的方法对环境保护、人类健康具有十分重要的意义。电致化学发光(ECL)技术以其低背景信号、高灵敏度和良好的可控性而受到广泛关注。近年来,基于汞离子极易被富含胸腺嘧啶的核酸捕获,形成“胸腺嘧啶-汞离子-胸腺嘧啶”(T-Hg-T)的稳定结构的特性,研究者们构建了针对汞离子的高选择性和稳定性的电致化学发光生物传感器。为了进一步提高该类生物传感器的性能,研究者们推陈出新了各种不同的信号输出模式,并显示出了各自的优势。该文针对汞离子的ECL生物分析传感应用研究,以各种不同的信号输出模式为类别,详述了近年来的研究进展,并对汞离子检测的ECL分析技术的发展做出了展望。

Mercury ion(Hg^(2+))is one of the most dangerous heavy metal pollution.It is easily transferred from the environment to the food chain and accumulates in the human body,causing serious damage to the brain,nervous system,endocrine system and kidneys.The development of fast,sensitive and accurate quantitative detection methods for Hg^(2+) is of great significance to environmental protection and human health.Electrochemiluminescence(ECL)has received wide attention for its low background signal,high sensitivity and good controllability.In recent years,based on the fact that mercury ions are easily captured by thymine-rich nucleic acids to form a stable structure of"T-Hg^(2+)-T",researchers have constructed ECL biosensors for mercury ions.In order to further improve the performance of biosensors,researchers have constructed a variety of different signal output modes,and show their respective advantages.This article reviews the latest sensing applications of ECL bioanalysis for mercury ions,summarizes various signal output modes of ECL bioanalysis,and looks forward to the development of mercury ion detection in ECL analysis technology.