灵芝子实体、菌丝体和孢子黑色素的结构表征与理化性质研究

Structural Characterization,Physicochemical Properties of Melanin from Fruiting Body,Hyphae and Spores of Ganoderma lucidum

为探讨灵芝子实体(GLM)、菌丝体(GLHM)和孢子(GLSM)黑色素结构和理化性质的差异,通过元素分析、紫外-可见光谱和傅里叶红外光谱等手段分析这3种黑色素的结构,并对这3种黑色素的理化性质如溶解性、色价、稳定性及体外抗氧化活性和对胰脂肪酶活性的抑制作用进行了比较研究。结果表明,这3种黑色素为真黑色素。与灵芝子实体和孢子黑色素相比,灵芝菌丝体黑色素具有更好的溶解性、色价和稳定性以及具有较强的体外抗氧化活性和体外对胰脂肪酶活性的抑制作用。研究结果为灵芝菌丝体黑色素的生物活性和实际应用提供理论基础。

In order to explore the difference in structure and physicochemical properties of melanin in fruiting body(GLM),hyphae(GLHM)and spores(GLSM)of G.lucidum,three melanin’s structures were analyzed by elemental analysis,UV-Vis and FT-IR,and the physicochemical properties including solubility,color value and stability,antioxidant activity and inhibitory effect on pancreatic lipase activity in vitro were compared.The results showed that three melanin were eumelanin.Compared with GLM and GLSM,GLHM presented better solubility,color value and stability,as well as stronger antioxidant activity and stronger inhibitory effect on pancreatic lipase activity in vitro.This study will provide a theoretical basis for the bioactivity and practical application of melanin from G.lucidum hyphae.