等离子体诱变选育高产胞外多糖花脸香蘑菌株

Breeding of Lepista sordida with High-yield Exopolysaccharide by Plasma Mutagenesis

花脸香蘑多糖具有较大的药用价值,选育高产多糖花脸香蘑菌株可丰富种质资源并促进花脸香蘑多糖科技创新和产业发展。以花脸香蘑原生质体单核体菌株LS01_10作为出发菌株,利用等离子体诱变技术选育高产胞外多糖的花脸香蘑菌株。在诱变功率为200 W,诱变时间为60 s的条件下,共获得5个性状稳定遗传的高产胞外多糖诱变菌株,其胞外多糖的产量是出发菌株的1.2-2.4倍。诱变菌株的菌丝形态和颜色都发生不同程度改变,且生物量均高于出发菌株。ISSR实验结果表明,诱变菌株与出发菌株相比在遗传水平发生不同程度的改变。高产多糖诱变菌株为进一步解析多糖高产机制提供了重要的菌种资源。

The polysaccharide of Lepista sordida owns huge medicinal value,and breeding L.sordida strain with high-yield polysaccharide could not only enrich the germplasm resources,but also promote the technological innovation and industrial development.To obtain L.sordida strains with high-yield exopolysaccharide,the monokaryon strain LS01_10 derived from protoplast was used as parental strain and plasma mutagenesis was conducted.When the optimal mutagenesis power was 200 W and inducing time was 60 s,5 mutant strains with high-yield exopolysaccharide and stable inheritance were obtained.The exopolysaccharide of these 5 mutant strains was 1.2-2.4 times higher than that of the parental strain.The morphology and color of hyphae of the mutant strains varied at different degree,the biomass was higher than that of the parental strain.The variations in genetic level among mutant and parental strains were confirmed by inter-simple sequence repea(t ISSR)analysis.The mutant strains with high-yield polysaccharide provide important germplasm resource for further resolving the mechanism of high polysaccharide yield.