泰山野生蛹虫草优良菌株的筛选

Screening of Superior Strains Isolated from Wild Cordyceps militaris in Mountain Tai

为进一步深入挖掘泰山地区的蛹虫草资源,获得高产优质的优良菌株,以采集分离自泰山地区的14株野生蛹虫草菌株为实验材料,通过菌丝长速测定、菌落特征观察、出草试验及主要活性物质(虫草素、N6-(2-羟乙基)腺苷、虫草多糖、β-类胡萝卜素、叶黄素)的测定分析,并利用归一化算法对各菌株特性进行综合评价。最终筛选出2个综合性状优异的蛹虫草菌株和1个虫草素含量较高的菌株。2个综合性状较优良的菌株的子实体成熟周期均为46.3 d,子实体粗长,生物学效率分别为55.31%和58.08%,虫草素含量分别为5.43 mg/g和7.13 mg/g,腺苷含量分别为0.38 mg/g和0.56 mg/g,多糖含量分别为90.44 mg/g和84.68 mg/g;1个菌株产量较低,但虫草素含量高达15.14 mg/g,腺苷含量也较高,为0.66 mg/g。

In order to further dig the resources of Cordyceps militaris in Mountain Tai area and to obtain the superior strains of C.militaris with high yield and high quality,14 wild strains isolated from wild C.militaris in Mountain Tai were used as experimental materials,the mycelial growth rate,colony characteristics,fruiting body morphology and yield,and the main active substance(s cordycepin,N6(-2-hydroxyethyl)adenosine,polysaccharide,β-carotenoid,and lutein)were determined,and the characteristics of each strain were evaluated by the normalization method.Finally,2 strains with superior comprehensive characteristics and 1 strain with high cordycepin content were selected.The selected two superior strains had the best comprehensive quality,the cultivation periods of fruit body were both 46.3 d,fruit bodies were thick and long,the biological efficiency was 55.31%and 58.08%,the cordycepin content was 5.43 mg/g and 7.13 mg/g,the adenosine content was 0.38 mg/g and 0.56 mg/g,the polysaccharide content was 90.44 mg/g and 84.68 mg/g,respectively.The high cordycepin content strain had lower yield,but the cordycepin content was up to 15.14 mg/g and adenosine content was also high(0.66 mg/g).