雨生红球藻转录因子Whirly1的获得及其表达分析

Acquisition and Expression Analysis of the Transcription Factor Whirly1 from Haematococcus pluvialis

Whirly转录因子广泛存在于植物中,参与植物生长发育进程和逆境胁迫耐受,对其功能的研究有助于我们理解雨生红球藻响应胁迫的机制。因此,先根据雨生红球藻192.80转录组测序数据,分离克隆到一个Whirly转录因子序列,并对其结构及表达进行分析。该转录因子的cDNA序列为924 bp,基因组序列为2556 bp,由有5个外显子和4个内含子组成,命名为HpWhirly1。HpWhirly1预测由244个氨基酸组成,拥有Whirly转录因子的保守结构域。HpWhirly1在醋酸钠和水杨酸胁迫早期表达上调,在强光-醋酸钠联合胁迫和缺氮胁迫条件下表达下调,表明其在雨生红球藻受到胁迫时发挥一定的作用。以上研究结果将有助于进一步研究Whirly转录因子的功能及其在雨生红球藻响应胁迫时的作用。

Whirly transcription factors are found in plants ubiquitously,and play a role in plant growth and stress resistance.Uncovering the functions of Whirly transcription factors would help us understand the response mechanism to stress in Haematococcus pluvialis.Hence,a Whirly transcription factor named HpWhirly1 was isolated and cloned based on the transcriptome data of H.pluvialis 192.80 and its structure and expression were analyzed.The cDNA sequence of this transcription factor consisted of 924 bp and genome sequence was 2556 bp with 5 exons and 4 introns.Results showed that HpWhirly1 was predicted to contain 244 amino acids and had the conserved domain of Whirly transcription factor.Further analysis showed that the expression of HpWhirly1 was up-regulated during the early stage of sodium acetate and salicylic acid stresses,while it was down-regulated under strong light sodium acetate combined stress and nitrogen deficiency stress.The above results indicated that HpWhirly1 might play a role in H.pluvialis when facing stresses and could respond to them.The results of this study are conducive to further studying the functions of Whirly transcription factors and its effects on the stress response of H.pluvialis.