维氏气单胞菌新型sRNA N155的鉴定及功能研究

Identification and Function Study of a New Type of sRNA N155 from Aeromonas veronii

维氏气单胞菌(Aeromonas veronii,A.veronii)是一种新型病原体,既能感染鱼类,又可以感染包括人在内的哺乳动物,其致病机理尚不完全清楚。基于A.veronii C4野生型菌株转录组数据一个新的sRNA N155被鉴定,进一步利用同源重组等分子生物学手段构建了sRNA N155的缺失菌株。转录组数据和RT-qPCR分析结果表明,sRNA N155独立转录。序列比对分析发现,sRNA N155具有物种特异性,仅存在于气单胞菌属中。生长曲线测定和运动性实验结果表明,sRNA N155的敲除不影响A.veronii生长,但会导致A.veronii运动能力下降。本研究成功构建的ΔsRNA N155敲除菌株,为进一步研究sRNA N155在A.veronii中的功能提供了可靠材料。

Aeromonas veronii is a new type of pathogen that can infect both fish and mammals including humans.and its pathogenic mechanism is not fully understood.A new sRNA N155 in wild type A.veronii C4 was identified based on the transcriptome data,and molecular biological methods such as homologous recombination were used to construct the sRNA N155 deletion strain.The results of transcriptome data and RT-qPCR analysis showed that sRNA N155 was independently transcribed.Sequence alignment analysis indicated that sRNA N155 was species-specific and only existed in Aeromonas.The results of growth curve determination and swimming motility experiments revealed that the knockout of sRNA N155 didn’t affect the growth of A.veronii,but caused the swimming motility of A.veronii reduced.TheΔsRNA N155 strain successfully constructed in this study provides a reliable material for further research on the function of sRNA N155 in A.veronii.