磷酸甘油酸变位酶1在单次热应激小鼠睾丸中的表达变化

Dynamic changes of the PGAM1 expression in the mouse testis exposed to single heat stress

目的:探讨磷酸甘油酸变位酶1(PGAM1)在单次热应激致小鼠睾丸中的表达变化。方法:C57雄性小鼠32只,随机分为对照组(n=16)和热应激组(n=16)。热应激组和对照组小鼠下腹部分别置于43℃和25℃水浴中干预15 min,于处理后1、7 d取睾丸组织。HE检测睾丸组织细胞形态改变,免疫组化染色检测PGAM1蛋白定位及表达,Western印迹检测PGAM1蛋白表达水平。结果:HE结果显示,在热应激后小鼠睾丸体积显著减小,生精小管结构紊乱,细胞数量减少,第7天后细胞基本消失。免疫组化显示,PGAM1蛋白在睾丸生精小管广泛表达,热应激1天后睾丸组织中PGAM1的表达显著高于对照组,第7天睾丸组织中PGAM1表达较第1天下调,但仍显著高于对照组。Western印迹检测显示,与对照组相比,热应激后PGAM1蛋白表达明显增加。结论:小鼠睾丸热应激处理后PGAM1蛋白表达量在睾丸中出现动态变化,这种变化与热应激后睾丸生精细胞的增殖分裂相关。

Objective:To investigate the expression of phosphoglycerate mutase 1(PGAM1)in the mouse testis after exposure to single heat stress(SHS).Methods:We randomly assigned 32 C57 male mice to an SHS(n=16)and a control group(n=16),the former bathed in water at 43℃and the latter at 25℃for 15 minutes.At 1 and 7 days after exposure,we harvested the testicular tissue for observation of the morphological changes of testicular cells by HE staining and determination of the location and expression of the PGAM1 protein by immunohistochemistry and Western blot.Results:The testis volume of the mice were reduced significantly,the spermatogenic tubules were disorganized,and the cells were reduced in number after heat stress and basically disappeared after 7 days.Immunohistochemistry showed extensive expression of the PGAM1 protein in the testicular spermatogenic tubules of the SHS-exposed mice,significantly higher than in the control group at 1 day after exposure,which was down-regulated in the testis tissue at 7 days,but still markedly higher than that in the control.Western blot exhibited significantly up-regulated expression of the PGAM1 protein after heat stress compared with that in the control group.Conclusion:The expression of the PGAM1 protein undergoes dynamic changes in the mouse testis after exposed to single heat stress,which is related to heat stress-induced proliferation and division of testicular spermatogenic cells.