大孔树脂分离纯化柞树叶总黄酮及其抗氧化活性研究

Study on Separation and Purification of Total Flavonoids from Tussah Leaves by Macroporous Resin and Its Antioxidant Activity

[目的]研究柞树叶总黄酮的纯化工艺及抗氧化活性。[方法]采用静态吸附与解析的方法,通过比较6种大孔树脂的吸附和解析性能,优选适宜的大孔树脂,并优化适合分离柞树叶总黄酮的解析条件。以V;为对照,对其还原力、DPPH自由基(DPPH·)和超氧阴离子自由基(O;·)的清除能力进行研究。[结果]D101型大孔树脂纯化柞树叶总黄酮吸附与洗脱效果最好,其纯化最佳工艺条件为粗提液浓度0.55 mg/mL、上样流速1.5 mL/min、洗脱剂40%乙醇(V/V)、洗脱流速1.5 mL/min,柞树叶总黄酮纯度可达65.5%;分离纯化后柞树叶总黄酮具有良好的总还原能力以及清除DPPH自由基和抗超氧阴离子自由基活性,其总还原力大于V;的还原力,当质量浓度为0.03 mg/mL时,对DPPH自由基和抗超氧阴离子自由基的清除率分别为85.9%和65.9%。[结论]D101大孔树脂可用于柞树叶总黄酮的分离纯化,柞树叶总黄酮具有较强的抗氧化活性。

[Objective]To study the purification technology and antioxidant activity of total flavonoids from tussah leaves.[Method]The suitable macroporous resin and the suitable desorption conditions for total flavonoids separation from tussah leaves were optimized by comparing the adsorption and desorption properties of six kinds of macroporous resin through static test.The reducing power,the scavenging ability of DPPH radical(DPPH·)and superoxide anion radical(O;·)were studied,as V;was control.[Result]The adsorption and elution effect of total flavonoids from tussah leaves by D101 macroporous resin was the best.The purity of total flavonoids in tussah leaves could reach 65.5%with the optimum purification conditions:crude extract concentration of 0.55 mg/mL,sample flow rate of 1.5 mL/min,elution agent of 40%ethanol(V/V),elution flow rate of 1.5 mL/min.After separation and purification,the total flavonoids of tussah leaves had good total reducing ability and scavenging DPPH free radical and anti-superoxide anion free radical activity,and its total reducing power was greater than that of V;.When the mass concentration was 0.03 mg/mL,the scavenging rates of DPPH free radical and anti-superoxide anion radical were 85.9%and 65.9%,respectively.[Conclusion]D101 macroporous resin can be used for the separation and purification of total flavonoids from tussah leaves,and the total flavonoids from tussah leaves have strong antioxidant activity.