UPLC-MS/MS法同时测定一清颗粒中5种成分的含量

Simultaneous Determination of Five Components in Yiqing granules by UPLC-MS/MS

目的:建立超高效液相色谱-串联质谱(UPLC-MS/MS)同时测定一清颗粒中大黄素、大黄酚、大黄素甲醚、黄芩苷及盐酸小檗碱的含量方法。方法:采用ACQUITY UPLC BEN C_(18)色谱柱(50 mm×2.1 mm,1.7μm),流动相为乙腈-水(含0.1%甲酸),梯度洗脱;电喷雾离子源(ESI),正、负离子切换,多反应监测模式(MRM)。结果:5种成分在对应的线性范围中具有良好的线性关系,样品回收率在86.13%~96.37%(n=6),RSD在2.19%~3.03%。结论:该方法操作简单,定量准确,灵敏度高,可适用于一清颗粒中5种成分的质量控制。

Objective To establish a method for the simultaneous determination of emodin,chrysophanol,physcion,baicalin and berberine hydrochloride in Yiqing granules by ultra performance liquid chromatography tandem mass spectrometry(UPLC-MS/MS).Methods The stationary phase was ACQUITY UPLC BEN C_(18) column(2.1 mmx50 mm,1.7 m),the mobile phase was acetonitrile-0.1%formic acid solution,gradient elution,electrospray ion source(ESI),positive and negative ions were detected simultaneously,and multi reaction monitoring mode(MRM).Results The linear range of the five components was good,the average recoveries(n=6)were 86.13%~96.37%,RSD were 2.19%~3.03%.Conclusion The method is simple,accurate and sensitive,and can be used for the quality control of 5 components in Yiqing granules.