春兰ב寒香梅’试管开花诱导及其生理基础

In vitro Flowering and Basic Physiological Research of Cymbidium goeringii ×Cymbidium ’Han Xiang Mei’

以春兰ב寒香梅’杂交种(Cymbidium goeringii×Cymbidium‘Han Xiang Mei’)为材料,MS+琼脂4 g·L^(-1)+蔗糖20 g·L^(-1)+椰汁100 mL·L^(-1)为基础培养基,通过单因素试验,探讨细胞分裂素(TDZ/6-BA)和无机盐浓度(P、K)对其试管花诱导的影响,测定花芽诱导的蛋白质、可溶性总糖含量与超氧化物歧化酶(SOD)活性及激素水平(IAA、ABA)。结果表明,添加0.2 mg·L^(-1)TDZ和2 mg·L^(-1)6-BA的花芽诱导率最高,分别为14.33%和14.00%;无机盐浓度3P/3K和3P/5K的培养基花芽诱导率较高,分别达16.67%和11.33%;花芽诱导最佳培养基为MS(3P,3K)+TDZ 0.2 mg·L^(-1)+椰汁100 mL·L^(-1)+琼脂4 g·L^(-1)+蔗糖20 g·L^(-1),花芽诱导率可达34%左右。生理生化指标检测显示,可溶性蛋白、可溶性总糖含量及SOD活性与花芽诱导率呈正相关;稳定的内源激素IAA和ABA含量对花芽诱导有一定的积极作用,含量过高对花芽诱导有抑制作用。

The study on the in vitro flowering of the hybrids Cymbidium goeringii×Cymbidium‘Han Xiang Mei’was implemented.The effects of cytokinin (TDZ/6-BA) and inorganic salt concentration (P,K) on the induction of flower in vitro were studied by single factor experiment with agar 4 g·L^(-1)+sucrose 20 g·L^(-1)+coconut milk100 m L·L^(-1)on the basis of MS medium physiological and biochemical indexes (protein,soluble total sugar,superoxide dismutase) and hormone (IAA,ABA) levels during the flower bud induction were measured.The results showed that the induction rate of flower bud was the highest with 0.2 mg·L^(-1)TDZ and 2 mg·L^(-1)6-BA,14.33%and14.00%respectively.The highest induction rates of 3P/3K and 3P/5K of inorganic salts were 16.67%and 11.33%respectively.The optimum medium caused the flower bud induction rate up to about 34%.The formula was as follows:MS (3P,3K)+TDZ 0.2 mg·L^(-1)+coconut milk 100 mL·L^(-1)+agar 4 g·L^(-1)+sucrose 20 g·L^(-1).Results of physiological and biochemical indexes showed that the contents of soluble protein,soluble total sugar and SOD activity were positively correlated with flower bud induction rate.The stability of endogenous hormone IAA and ABA content was beneficial to flower bud induction,but too much might inhibit the induction of flower buds.