盐胁迫下AM真菌对西瓜幼苗叶片防御酶和内源激素的影响

Effects of AM Fungus on Defense Enzymes and Endogenous Hormones in Leaves of Watermelon Seedlings under Salt Stress

为了探讨丛枝菌根(arbuscular mycorrhizal,AM)真菌对西瓜耐盐性的影响,以西瓜[Citrullus lanatus(Thunb.)Matsum et Nakai]品种‘京欣四号’为材料,研究了AM真菌对盐胁迫下西瓜幼苗叶片防御酶活性、脯氨酸及内源激素含量的影响。本试验共4个处理:对照(未盐胁迫且不接种AM真菌)、只进行盐胁迫、只接种AM真菌、盐胁迫且接种AM真菌。结果表明:盐胁迫下,接种AM真菌提高了西瓜幼苗叶片抗氧化系统中的超氧化物歧化酶和过氧化氢酶的活性,比未接种的处理提高了17.3%和5.4%;并增加叶片中脯氨酸、赤霉素和吲哚乙酸的含量,分别比对照增加了44.2%、22.9%和21.9%。结论认为接种AM真菌能够增强西瓜幼苗对盐胁迫的抗性。

In order to explore the effects of arbuscular mycorrhizal(AM)fungus improving watermelon salt tolerance,the defensive enzyme activity,proline and endogenous hormone contents in leaves of watermelon Citrullus Lanatus(Thunb.)Matsum et Nakai‘Jingxin 4’seedlings were studied under salt stress.There were four treatments in the experiment control(no salt stress and no inoculation of AM fungus),only salt stress,only inoculation of AM fungus,salt stress and inoculation of AM fungus.The results showed that under salt stress,the activity of superoxide dismutase(SOD)and catalase(CAT)in antioxidant system of watermelon seedling leaves were increased by 17.3%and 5.4%by AM fungi inoculation compared with that without AM fungi inoculation.The contents of proline,gibberellin and indoleacetic acid in leaves were increased by 44.2%,22.9%and 21.9%compared with that of control,respectively.It is concluded that inoculation with AM fungi can enhance the resistance of watermelon seedlings to salt stress.