基于GEO数据库筛查小鼠缺血性卒中后心脏组织的差异基因

Analysis of differential genes of ischemic stroke-induced heart tissue in mice based on GEO database

目的:分别比较缺血后24 h和72 h大脑中动脉闭塞模型(MCAO)小鼠与假手术(Sham)小鼠左心室的差异基因,分析缺血性卒中后不同时间点的差异基因及其调控的功能通路,以期分析缺血性卒中后诱发心功能障碍的机制并为其治疗提供依据。方法:在美国国立生物技术信息中心(NCBI)的GEO数据库下载MCAO小鼠与Sham小鼠左心室的基因芯片数据;通过R语言软件编程处理,获得差异表达基因;利用DAVID 6.8在线分析工具对获得的差异基因进行GO功能富集和KEGG通路富集分析,并运用Omicshare在线分析工具对富集分析结果进行可视化。结果:缺血后24 h,得到187个差异表达基因,56条具有显著意义的GO富集通路和5条具有显著意义的KEGG富集通路;缺血后72 h,得到51个差异表达基因,14条具有显著意义的GO富集通路和3条具有显著意义的KEGG富集通路。其中,两个时间点均涉及Aplnr、Itgb6两个基因靶点与PI3K-Akt信号通路。结论:本研究通过分析基因表达谱数据,得到缺血性卒中后诱导心功能障碍的差异表达基因及相关通路;PI3K-Akt信号通路与心功能调节密切相关,调节PI3K-Akt信号通路可能是治疗缺血性卒中后并发心功能障碍的重要方向。

Objective:To evulate the differential genes of left ventricle in the middle cevebral artery occlusion(MCAO)mice and Sham mice 24 h and 72 h after ischemia respectively,and to analyze the differential genes and their regulated functional pathways at different time points after ischemic stroke,so as to explore the mechanism of cardiac dysfunction induced by ischemic stroke and to provide evidence for its treatment.Methods:Gene-chip data from the left ventricle of MCAO mice and Sham mice were downloaded from the GEO database at the National Center for Biotechnology Information(NCBI).The differentially expressed genes were obtained by R language software programming.The GO functional enrichment and KEGG pathway enrichment analyses of the obtained differential genes were performed by using DAVID 6.8 online analysis tool,and the Omicshare online analysis tool was used to visualize the enrichment analysis results.Results:At 24 h after ischemia,187 differentially expressed genes were obtained,including 56 GO enrichment pathways and 5 KEGG enrichment pathways with significant significance.After 72 h after ischemia,51 differentially expressed genes were obtained,including 14 GO enrichment pathways and 3 KEGG enrichment pathways with significant significance.The two time points involved Aplnr and Itgb6 gene targets and PI3K-Akt signaling pathway.Conclusion:①By analyzing the gene expression profile data,the differentially expressed genes and related pathways of cardiac dysfunction induced by ischemic stroke are obtained.②PI3K-Akt signaling pathway is closely related to the regulation of cardiac function,and regulation of PI3K-Akt signaling pathway may be an important direction for the treatment of cardiac dysfunction after ischemic stroke.