根癌农杆菌介导真菌遗传转化的研究及应用

Research and application of Agrobacterium tumefaciens-mediated fungal genetic transformation

根癌农杆菌介导转化法(Agrobacterium tumefaciens-Mediated Transformation,ATMT)具有转化效率高、遗传稳定、适用范围广等诸多优点,已成为真菌遗传转化研究中的强有力手段,在真菌基因资源开发、真菌性疾病研究和外源蛋白表达研究中发挥巨大作用。本文概述了根癌农杆菌转化法在真菌转化中的研究进展、技术优缺点、转化机制、实验方法和应用现状,着重介绍影响其转化效率的因素并对优化方法进行探讨,展望了该技术在真菌基因资源发掘、基因编辑等方面的应用前景,为今后真菌的遗传转化研究提供参考。

Agrobacterium tumefaciens-mediated transformation(ATMT) has many advantages such as high transformation efficiency, genetic stability, and a wide range of applications. It has become a powerful method in the study of fungal genetics. It plays a huge role in fungal disease research and heterologous protein expression research. This article summarizes the research progress, technical advantages and disadvantages, transformation mechanism, experimental methods and application status of ATMT technology in fungi studying. This review also focuses on the influencing factors of ATMT transformation efficiency and discusses the optimization methods. The application prospects of genome editing and other aspects are expected to provide references for future fungal genetic transformation research.