顺式和反式-AT型聚酮合酶研究进展及应用

Research progress and application of cis-AT and trans-AT polyketide synthase

聚酮类化合物因广泛应用于医药等方面而被大家所熟知,Ⅰ型聚酮合酶(Polyketide Synthase,PKS)在催化聚酮类化合物的生物合成中起着重要的作用。根据不同的酰基转移酶(Acyltransferase,AT)结构域,Ⅰ型 PKS 可分为顺式-AT (cis-Acyltransferase,cis-AT)型 PKS 和反式-AT (trans-Acyltransferase,trans-AT)型PKS,目前cis-AT型PKS研究得比较透彻,trans-AT型PKS相关研究成为当今热点。本文总结了cis-AT型PKS和trans-AT型PKS的联系与区别、工程进展、相关应用以及目前存在的问题,以期为了解cis-AT型PKS和trans-AT型PKS在聚酮化合物合成中的作用提供参考。

Type I polyketide synthases(PKS) play an important role in catalyzing the biosynthesis of polyketides, which is widely used in medicine and other fields. According to different acyltransferase(AT)domains, type I PKS can be divided into cis-Acyltransferase(cis-AT) PKS and trans-Acyltransferase(trans-AT) PKS, the former has been studied more thoroughly at present, and the related research of the latter has become a hot spot. In this paper, the connections and differences between cis-AT PKS and trans-AT PKS, as well as engineering progresses, related applications and currently existing problems are reviewed, to provide a reference for understanding the role of cis-AT PKS and trans-AT PKS in the biosynthesis of polyketides.