五味甘露药浴颗粒对佐剂性关节炎大鼠药效及血清FGF、EGF、PDGF含量的影响

Effect of Wuwei Ganlu Medicine Bath Granules on drug efficacy and serum EGF,FGF and PDGF in adjuvant arthritis rats

目的:观察五味甘露药浴颗粒对佐剂关节炎大鼠的关节形态及对血清中成纤维细胞生长因子(FGF)、表皮细胞生长因子(EGF)、血小板衍生生长因子(PDGF)含量的影响,探索藏药浴法治疗类风湿性关节炎的作用机理。方法:以佐剂性关节炎(Adjuvant Arthritis Rats, AA)大鼠作为疾病模型,随机分为模型对照组、地塞米松组、五味甘露药浴颗粒高剂量组(11.80 g﹒L;)、中剂量组(5.90 g﹒L;)、低剂量组(2.95 g﹒L;)。致炎两周后,大鼠分别采用淡温水浴(40℃,30 min)、藏药浴(40℃,30 min)、地塞米松灌胃。连续给药四周后,用致炎后足跖肿胀度、HE染色法观察致炎侧足踝关节滑膜病理改变,评价藏药五味甘露药浴颗粒对AA大鼠的治疗效果,酶联免疫法测定大鼠血清中FGF、EGF、PDGF水平。结果:关节炎经温水浴治疗没有明显改变,而藏药浴低、中剂量组(2.95 g﹒L;、5.90 g﹒L;)和地塞米松组足肿胀程度均降低,关节炎性细胞浸润和软骨的破坏均减轻。中剂量组(5.90 g﹒L;)血清中FGF、PDGF水平显著下降(P<0.01),EGF水平明显下降(P<0.05)。结论:藏药五味甘露药浴颗粒作用于大鼠佐剂性关节炎疗效显著,能有效减小佐剂性关节炎大鼠致炎侧足跖肿胀度,改善AA大鼠踝关节滑膜病变,其机制可能与降低血清中FGF、EGF、PDGF的水平有关。

Objective: To observe the effects of Wuwei Ganlu Medicine Bath Granules on the joint morphology of adjuvant arthritis rats and the contents of fibroblast growth factor(FGF), epidermal cell growth factor(EGF) and platelet derived growth factor(PDGF) in serum, and to explore the mechanism of the treatment of rheumatoid arthritis with herbal bath. Method: Adjuvant Arthritis Rats(AA) were used as disease model and randomly divided into model control group, dexamethasone group, high-dose(11.80 g﹒L-1), medium-dose( 5.90 g﹒L;) and low-dose(2.95 g﹒L;) Wuwei Ganlu Medicine Bath Granules group. Two weeks after inflammation, the rats were given intragastric gavage with mild temperature water bath(40℃, 30 min), herbal bath(40℃, 30 min) and dexamethasone, respectively. After four weeks of continuous administration, swelling degree of the post-inflammatory voix pedis and pathological changes of synovium of the inflammatory ankle joint observed by HE staining were used to evaluate the therapeutic effect of Tibetan medicine Wuwei Ganlu Medicine Bath Granules on AA rats. Serum levels of EGF, FGF and PDGF were determined by enzyme-linked immunoassay. Result: Warm water had no effect on AA rat. The degree of foot swelling in the herbal bath of low dose, medium dose groups and dexamethasone groups was decreased. Both inflammatory cell infiltration and cartilage destruction were reduced. The serum levels of FGF and PDGF in medium-dose group were significantly decreased(P < 0.01), and the serum level of EGF was significantly decreased(P < 0.05). Conclusion: Tibetan medicine Wuwei Ganlu Medicine Bath Granules has a significant effect on AA. It can effectively reduce the swelling degree of the inflammatory voix pedis and improve the synovial lesions of the ankle in AA rats. The mechanism may be related to the decrease of serum levels of EGF, FGF and PDGF.