自身免疫抗体在肺结核患者中的检出率及与肺功能的相关性研究

Detection rate of autoimmune antibody in patients with pulmonary tuberculosis and its correlation with pul-monary function

目的探讨自身免疫抗体在肺结核患者中的检出率及与肺功能的相关性。方法选择2020年6月至12月肺结核患者646例为观察组;选择同期健康体检者120名为对照组。采用荧光显微镜和全自动膜条仪测定抗核抗体(ANA)、抗中性粒细胞细胞质抗体(ANCA)及ANA 15项、ANCA 3项;采用肺功能测定仪完成患者肺功能第1秒用力呼气量(FEV_(1))、用力肺活量(FVC)、第1秒用力呼气量占用力肺活量百分率(FEV_(1)/FVC);采用Pearson相关分析对肺结核患者自身免疫抗体与肺功能进行相关分析。结果646例肺结核患者均完成自身免疫抗体检测,结果显示:94例自身免疫抗体检测阳性,阳性率为14.6%。自身免疫抗体中主要为ANCA抗体、抗蛋白酶3(PR3)抗体、抗SSB抗体,分别占17.0%、13.8%、12.8%;观察组肺结核患者肺功能水平均低于对照组(P<0.05);肺结核患者中自身免疫抗体、抗ANA抗体、抗髓过氧化物酶(MPO)抗体与抗SSB抗体、抗SSA抗体与肺功能FEV_(1)、FVC、FEV_(1)/FVC水平呈负相关(P<0.05)。结论自身免疫抗体在肺结核患者中具有一定的检出率,且与肺功能水平存在相关性,能反映患者疾病严重程度,可指导临床诊疗。

Objective To investigate the detection rate of autoimmune antibodies in patients with pulmonary tuberculosis and its correlation with pulmonary function.Methods Six hundred and forty-six patients with pulmonary tuberculosis from June 2020 to December 2020 were selected as the observation group,and 120 healthy people were selected as the control group.The antinuclear antibody(ANA),antineutrophil cytoplasmic antibody(ANCA),ANA 15 and ANCA 3 were measured by fluorescence microscope and automatic membrane strip instrument;the forced expiratory volume in the first second(FEV_(1)),forced vital capacity in the first second(FVC)and the percentage of forced expiratory volume in the first second(FEV_(1)/FVC)were measured by lung function analyzer;Pearson correlation analysis was used to analyze the correlation between pulmonary function and autoimmune antibody in patients with pulmonary tuberculosis was analyzed.Results Six hundred and forty-six cases of pulmonary tuberculosis patients were completed autoimmune antibody test,the results showed that:94 cases of autoimmune antibody test positive,the positive rate was 14.6%.ANCA antibody,anti PR3 antibody and anti SSB antibody were the main autoimmune antibodies,accounting for 17.0%,13.8%and 12.8%respectively;the pulmonary function level of tuberculosis patients in the observation group was lower than that of the control group(P<0.05);the results showed that the levels of ANA antibody,anti-myeloperoxidase(MPO)antibody and anti SSB antibody,anti SSA and pulmonary function FEV_(1),FVC,FEV_(1)/FVC in tuberculosis patients were negatively correlated(P<0.05).Conclusion Autoimmune antibody has a certain detection rate in patients with pulmonary tuberculosis,and has a correlation with the level of pulmonary function,which can reflect the severity of the disease and guide clinical diagnosis and treatment.