一种过氧化氢低温消毒液的杀菌效果研究

Study on the germicidal effect of a low-temperature disinfectant of hydrogen peroxide

目的观察一种过氧化氢低温消毒液的杀菌效果。方法采用载体浸泡定量杀菌试验方法,对该过氧化氢低温消毒液杀灭效果进行观察。结果含乙醇防冻剂的16 g/L过氧化氢消毒液在20℃条件下,对载体上大肠埃希菌、金黄色葡萄球菌和白色念珠菌分别作用0.5、1.0和2.0 min,杀灭对数值均>3.00。含乙醇防冻剂的16 g/L过氧化氢消毒液在-20℃条件下,对载体上金黄色葡萄球菌和大肠埃希菌作用30 min,杀灭对数值均>3.00;对载体上白色念珠菌作用40 min,杀灭对数值>3.00。结论该过氧化氢低温消毒液在低温(-20℃)条件下可有效杀灭细菌繁殖体和真菌。

Objective To observe the germicidal effect of a low-temperature hydrogen peroxide disinfectant.Methods Carrier immersion quantitative germicidal test was used to observe the killing effect of the low-temperature hydrogen peroxide disinfectant.Results Under the condition of 20℃,the 16 g/L hydrogen peroxide disinfectant containing ethanol as an antifreeze acted on Escherichia coli,Staphylococcus aureus and Candida albicans on the carrier for 0.5,1.0 and 2.0 min,respectively,and the killing logarithmic values were all>3.00.Under the condition of-20℃,the 16 g/L hydrogen peroxide disinfectant containing ethanol as an antifreeze acted on Staphylococcus aureus and Escherichia coli on the carrier for 30 min,and the killing logarithmic values were both>3.00;acted on Candida albicans on the carrier for 40 min,and the killing logarithmic value was>3.00.Conclusion The low-temperature hydrogen peroxide disinfectant can effectively kill bacterial propagules and fungi under the condition of low temperature(-20℃).