双酶法制备羊栖菜多肽的工艺研究

Study on Preparation of Sargassum Fusiforme Polypeptide by Double Enzyme Method

本试验采用双酶法(碱性蛋白酶+木瓜蛋白酶)制备羊栖菜多肽,研究了酶与底物浓度比、酶解温度、pH和酶解时间对水解度的影响。通过响应面设计,结果表明双酶法B(碱性蛋白酶∶木瓜蛋白=1∶1)水解羊栖菜蛋白的最佳酶解条件为酶底比为6%、酶解温度60℃、pH为6.5、酶解时间180 min、水解度为41.73%。

In this experiment,sargassum fusiforme polypeptide was prepared by double enzyme method(alkaline protease+papain).The effects of enzyme substrate concentration ratio,enzymatic hydrolysis temperature,pH and enzymatic hydrolysis time on the degree of hydrolysis were studied.Through response surface design,the results showed that the optimum enzymatic hydrolysis conditions of sargassum fusiforme protein hydrolyzed by double enzyme method B(alkaline protease∶papain=1∶1)were as follows:enzyme substrate ratio was 6%,enzymatic hydrolysis temperature was 60℃,pH was 6.5,enzymatic hydrolysis time was 180 min,and the degree of hydrolysis was 41.73%.